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PMID: 2684655 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Functional compartments of the yeast Golgi apparatus are defined by the sec7 mutation.

The EMBO journal ·Vol. 8 ·No. 9 ·1989-09-00 ·Pages 2695-702

Franzusoff A, Schekman R

Abstract

The role of the SEC7 gene product in yeast intercompartmental protein transport was examined. A spectrum of N-linked oligosaccharide structures, ranging from core to nearly complete outer chain carbohydrate, was observed on glycoproteins accumulated in secretion-defective sec7 mutant cells. Terminal alpha 1-3-linked outer chain mannose residues failed to be added to N-linked glycoproteins in sec7 cells at the restrictive temperature. These results suggest that outer chain glycosyl modifications do not occur within a single compartment. Additional evidence consistent with subdivision of the yeast Golgi apparatus came from a cell-free glycoprotein transport reaction in which wild-type membranes sustained outer chain carbohydrate growth up to, but not including, addition of alpha 1-3 mannose residues. Golgi apparatus compartments may specialize in addition of distinct outer chain determinants. The SEC7 gene product was suggested to regulate protein transport between and from functional compartments of the yeast Golgi apparatus.

MeSH Terms
Biological Transport/genetics,physiology Endoplasmic Reticulum/metabolism Fungal Proteins/metabolism Glycoproteins/metabolism Glycoside Hydrolases/metabolism Glycosylation Golgi Apparatus/physiology Mating Factor Mutation Peptides/metabolism Protein Precursors/metabolism Saccharomyces cerevisiae/genetics,metabolism beta-Fructofuranosidase
Chemicals
Fungal Proteins Glycoproteins Peptides Protein Precursors Mating Factor Glycoside Hydrolases beta-Fructofuranosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Franzusoff A
Department of Biochemistry, University of California, Berkeley 94720.
Schekman R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-09-00
Pages
2695-702
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401276
Subset
IM
Grants
NIGMS NIH HHS · GM10673 · United States
NIGMS NIH HHS · GM26755 · United States
NIGMS NIH HHS · GM36881 · United States
External Links
PubMed source PMC Full Text View journal details
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