Home LiteratureArticle Details
PMID: 9891079 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cyclin E associates with BAF155 and BRG1, components of the mammalian SWI-SNF complex, and alters the ability of BRG1 to induce growth arrest.

Molecular and cellular biology ·Vol. 19 ·No. 2 ·1999-02-00 ·Pages 1460-9

Shanahan F, Seghezzi W, Parry D, Mahony D, Lees E

Abstract

SWI-SNF complexes have been implicated in transcriptional regulation by chromatin remodeling. We have identified an interaction between two components of the mammalian SWI-SNF complex and cyclin E, an essential cell cycle regulatory protein required for G1/S transition. BRG1 and BAF155, mammalian homologs of yeast SWI2 and SWI3, respectively, are found in cyclin E complexes and are phosphorylated by cyclin E-associated kinase activity. In this report, we show that overexpression of BRG1 causes growth arrest and induction of senescence-associated beta-galactosidase activity, which can be overcome by cyclin E. Our results suggest that cyclin E may modulate the activity of the SWI-SNF apparatus to maintain the chromatin in a transcriptionally permissive state.

MeSH Terms
Amino Acid Sequence Cell Cycle Cell Line Cell Size Cyclin E/genetics,metabolism DNA Helicases DNA-Binding Proteins/genetics,metabolism Fungal Proteins/genetics,metabolism Humans Molecular Sequence Data Nuclear Proteins/genetics,metabolism Phenotype Retinoblastoma Protein/metabolism Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors/genetics,metabolism Transfection
Chemicals
Cyclin E DNA-Binding Proteins Fungal Proteins Nuclear Proteins Retinoblastoma Protein SMARCA2 protein, human SMARCC1 protein, human SWI3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors SMARCA4 protein, human DNA Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Shanahan F
Cell Signaling Department, DNAX Research Institute, Palo Alto, California 94304, USA.
Seghezzi W
Parry D
Mahony D
Lees E
References (57)
57 references, click to expand
  1. Association of human cyclin E with a periodic G1-S phase protein kinase.
    Science. 1992 Sep 25;257(5078):1958-61 PMID: 1329201
  2. Regulation of retinoblastoma protein functions by ectopic expression of human cyclins.
    Cell. 1992 Sep 18;70(6):993-1006 PMID: 1388095
  3. Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle.
    Science. 1992 Sep 18;257(5077):1689-94 PMID: 1388288
  4. Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner.
    Genes Dev. 1992 Oct;6(10):1874-85 PMID: 1398067
  5. Interaction of p107 with cyclin A independent of complex formation with viral oncoproteins.
    Science. 1992 Jan 3;255(5040):85-7 PMID: 1532457
  6. Interaction between human cyclin A and adenovirus E1A-associated p107 protein.
    Science. 1992 Jan 3;255(5040):87-90 PMID: 1532458
  7. Nonfunctional mutants of the retinoblastoma protein are characterized by defects in phosphorylation, viral oncoprotein association, and nuclear tethering.
    Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3033-7 PMID: 1826560
  8. G1/S phosphorylation of the retinoblastoma protein is associated with an altered affinity for the nuclear compartment.
    Cell. 1991 May 3;65(3):381-93 PMID: 2018973
  9. Suppression of the neoplastic phenotype by replacement of the RB gene in human cancer cells.
    Science. 1988 Dec 16;242(4885):1563-6 PMID: 3201247
  10. Activation of the phosphatase activity of human cdc25A by a cdk2-cyclin E dependent phosphorylation at the G1/S transition.
    EMBO J. 1994 Sep 15;13(18):4302-10 PMID: 7523110
  11. A biomarker that identifies senescent human cells in culture and in aging skin in vivo.
    Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9363-7 PMID: 7568133
  12. Human cyclin E, a nuclear protein essential for the G1-to-S phase transition.
    Mol Cell Biol. 1995 May;15(5):2612-24 PMID: 7739542
  13. A human protein with homology to Saccharomyces cerevisiae SNF5 interacts with the potential helicase hbrm.
    Nucleic Acids Res. 1995 Apr 11;23(7):1127-32 PMID: 7739891
  14. The SWI-SNF complex: a chromatin remodeling machine?
    Trends Biochem Sci. 1995 Apr;20(4):143-6 PMID: 7770913
  15. Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5.
    Science. 1994 Dec 23;266(5193):2002-6 PMID: 7801128
  16. Principles of CDK regulation.
    Nature. 1995 Mar 9;374(6518):131-4 PMID: 7877684
  17. The SNF/SWI family of global transcriptional activators.
    Curr Opin Cell Biol. 1994 Jun;6(3):396-402 PMID: 7917331
  18. The retinoblastoma protein and BRG1 form a complex and cooperate to induce cell cycle arrest.
    Cell. 1994 Oct 7;79(1):119-30 PMID: 7923370
  19. E2F-4, a new member of the E2F transcription factor family, interacts with p107.
    Genes Dev. 1994 Nov 15;8(22):2665-79 PMID: 7958924
  20. Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex.
    Science. 1994 Jul 1;265(5168):53-60 PMID: 8016655
  21. Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals.
    Cell. 1994 Jul 15;78(1):59-66 PMID: 8033212
  22. Nucleosome disruption and enhancement of activator binding by a human SW1/SNF complex.
    Nature. 1994 Aug 11;370(6489):477-81 PMID: 8047169
  23. Facilitated binding of TATA-binding protein to nucleosomal DNA.
    Nature. 1994 Aug 11;370(6489):481-5 PMID: 8047170
  24. Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system.
    Mol Cell Biol. 1994 Mar;14(3):1669-79 PMID: 8114703
  25. Cdc25A is a novel phosphatase functioning early in the cell cycle.
    EMBO J. 1994 Apr 1;13(7):1549-56 PMID: 8156993
  26. Two human homologues of Saccharomyces cerevisiae SWI2/SNF2 and Drosophila brahma are transcriptional coactivators cooperating with the estrogen receptor and the retinoic acid receptor.
    Nucleic Acids Res. 1994 May 25;22(10):1815-20 PMID: 8208605
  27. A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor.
    EMBO J. 1993 Nov;12(11):4279-90 PMID: 8223438
  28. BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription.
    Nature. 1993 Nov 11;366(6451):170-4 PMID: 8232556
  29. Distinct roles for cyclin-dependent kinases in cell cycle control.
    Science. 1993 Dec 24;262(5142):2050-4 PMID: 8266103
  30. Functional interactions of the retinoblastoma protein with mammalian D-type cyclins.
    Cell. 1993 May 7;73(3):487-97 PMID: 8343202
  31. The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2.
    J Virol. 1993 May;67(5):2456-65 PMID: 8386263
  32. Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4.
    Genes Dev. 1993 Mar;7(3):331-42 PMID: 8449399
  33. Physical interaction of the retinoblastoma protein with human D cyclins.
    Cell. 1993 May 7;73(3):499-511 PMID: 8490963
  34. The cdk2 kinase is required for the G1-to-S transition in mammalian cells.
    Oncogene. 1993 Jun;8(6):1593-602 PMID: 8502482
  35. Cyclin dependent kinase regulation.
    Curr Opin Cell Biol. 1995 Dec;7(6):773-80 PMID: 8608007
  36. Functional interactions between the hBRM/hBRG1 transcriptional activators and the pRB family of proteins.
    Mol Cell Biol. 1996 Apr;16(4):1576-83 PMID: 8657132
  37. Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex.
    Science. 1996 Jul 26;273(5274):513-6 PMID: 8662543
  38. The hbrm and BRG-1 proteins, components of the human SNF/SWI complex, are phosphorylated and excluded from the condensed chromosomes during mitosis.
    EMBO J. 1996 Jul 1;15(13):3394-402 PMID: 8670841
  39. Diversity and specialization of mammalian SWI/SNF complexes.
    Genes Dev. 1996 Sep 1;10(17):2117-30 PMID: 8804307
  40. Purification and biochemical heterogeneity of the mammalian SWI-SNF complex.
    EMBO J. 1996 Oct 1;15(19):5370-82 PMID: 8895581
  41. Cancer cell cycles.
    Science. 1996 Dec 6;274(5293):1672-7 PMID: 8939849
  42. RSC, an essential, abundant chromatin-remodeling complex.
    Cell. 1996 Dec 27;87(7):1249-60 PMID: 8980231
  43. Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression.
    Mol Cell Biol. 1997 Jun;17(6):3323-34 PMID: 9154831
  44. Cyclin E-induced S phase without activation of the pRb/E2F pathway.
    Genes Dev. 1997 Jun 1;11(11):1479-92 PMID: 9192874
  45. Bypass of senescence after disruption of p21CIP1/WAF1 gene in normal diploid human fibroblasts.
    Science. 1997 Aug 8;277(5327):831-4 PMID: 9242615
  46. S-Phase entry upon ectopic expression of G1 cyclin-dependent kinases in the absence of retinoblastoma protein phosphorylation.
    Curr Biol. 1997 Sep 1;7(9):709-12 PMID: 9285720
  47. Cyclin E and c-Myc promote cell proliferation in the presence of p16INK4a and of hypophosphorylated retinoblastoma family proteins.
    EMBO J. 1997 Sep 1;16(17):5322-33 PMID: 9311992
  48. RB and hbrm cooperate to repress the activation functions of E2F1.
    Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11268-73 PMID: 9326598
  49. Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays.
    EMBO J. 1997 Nov 17;16(22):6772-82 PMID: 9362491
  50. Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry.
    Genes Dev. 1998 Feb 15;12(4):456-61 PMID: 9472014
  51. Inhibitors of cyclin-dependent kinases induce features of replicative senescence in early passage human diploid fibroblasts.
    Curr Biol. 1998 Mar 12;8(6):351-4 PMID: 9512419
  52. Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis.
    Genes Dev. 1998 May 15;12(10):1409-14 PMID: 9585501
  53. Truncating mutations of hSNF5/INI1 in aggressive paediatric cancer.
    Nature. 1998 Jul 9;394(6689):203-6 PMID: 9671307
  54. Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells.
    Mol Cell Biol. 1998 Aug;18(8):4526-36 PMID: 9671462
  55. Human SWI/SNF interconverts a nucleosome between its base state and a stable remodeled state.
    Cell. 1998 Jul 10;94(1):17-27 PMID: 9674423
  56. Activated RSC-nucleosome complex and persistently altered form of the nucleosome.
    Cell. 1998 Jul 10;94(1):29-34 PMID: 9674424
  57. Mitotic inactivation of a human SWI/SNF chromatin remodeling complex.
    Genes Dev. 1998 Sep 15;12(18):2842-51 PMID: 9744861
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.19.2.1460
Published
1999-02-00
Pages
1460-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC116074
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.19.2.1460 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2