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PMID: 9585501 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis.

Genes & development ·Vol. 12 ·No. 10 ·1998-05-15 ·Pages 1409-14

Wang C, Chua K, Seghezzi W, Lees E, Gozani O, Reed R

Abstract

The U2 snRNP component SAP 155 contacts pre-mRNA on both sides of the branch site early in spliceosome assembly and is therefore positioned near or at the spliceosome catalytic center. We have isolated a cDNA encoding human SAP 155 and identified its highly related Saccharomyces cerevisiae homolog (50% identity). The carboxy-terminal two-thirds of SAP 155 shows the highest conservation and is remarkably similar to the regulatory subunit A of the phosphatase PP2A. Significantly, SAP 155 is phosphorylated concomitant with or just after catalytic step one, making this the first example of a protein modification tightly regulated with splicing catalysis.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Binding Sites Caenorhabditis elegans/genetics Catalysis Consensus Sequence DNA, Complementary/genetics Genes, Fungal Helminth Proteins/chemistry Humans Macromolecular Substances Molecular Sequence Data Phosphoprotein Phosphatases/chemistry Phosphoproteins/genetics,metabolism Phosphorylation Protein Binding Protein Processing, Post-Translational RNA Precursors/metabolism RNA Splicing/physiology RNA Splicing Factors Ribonucleoprotein, U2 Small Nuclear/genetics,metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Schizosaccharomyces/genetics Sequence Alignment Sequence Homology, Amino Acid Spliceosomes/metabolism
Chemicals
Adaptor Proteins, Signal Transducing DNA, Complementary Helminth Proteins Macromolecular Substances Phosphoproteins RNA Precursors RNA Splicing Factors Ribonucleoprotein, U2 Small Nuclear SAP155 protein, S cerevisiae SF3B1 protein, human Saccharomyces cerevisiae Proteins Phosphoprotein Phosphatases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wang C
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.pende014@mc.duke.edu
Chua K
Seghezzi W
Lees E
Gozani O
Reed R
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.10.1409
Email
usa.pende014@mc.duke.edu
Published
1998-05-15
Pages
1409-14
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316838
Subset
IM
Databases
GENBANK
AF054284
External Links
PubMed source DOI: 10.1101/gad.12.10.1409 PMC Full Text View journal details
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