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PMID: 9864316 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Multiple small heat shock proteins in rhizobia.

Journal of bacteriology ·Vol. 181 ·No. 1 ·1999-01-00 ·Pages 83-90

Münchbach M, Nocker A, Narberhaus F

Abstract

Seven genes coding for small heat shock proteins (sHsps) in Bradyrhizobium japonicum have been identified. They are organized in five operons that are coordinately regulated by ROSE, a negatively cis-acting DNA element. The deduced sHsps can be divided into two separate classes: class A, consisting of proteins that show similarity to Escherichia coli IbpA and IbpB, and class B, whose members display significant similarity to other sHsps from prokaryotes and eukaryotes. Two-dimensional gel electrophoresis and Edman sequencing revealed the presence of at least 12 sHsps in B. japonicum, indicating a remarkable abundance of sHsps in this organism. Three additional members of class A and two potentially novel heat shock proteins were identified on the basis of their amino termini. The presence of multiple sHsps was also demonstrated for a variety of Rhizobium and Bradyrhizobium species by immunoblot analysis and two-dimensional gel electrophoresis. An extensive database survey revealed that, in contrast to the rhizobia, other bacteria contain maximally two sHsps whereas many plants have been reported to possess a sHsp superfamily.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/classification,genetics,isolation & purification Base Sequence DNA Primers/genetics Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation, Bacterial Genes, Bacterial Heat-Shock Proteins/classification,genetics,isolation & purification Hot Temperature Molecular Sequence Data Operon Rhizobiaceae/chemistry,genetics Rhizobium/chemistry,genetics Sequence Homology, Amino Acid Species Specificity
Chemicals
Bacterial Proteins DNA Primers Heat-Shock Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Münchbach M
Protein Chemistry Laboratory, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland.
Nocker A
Narberhaus F
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.1.83-90.1999
Published
1999-01-00
Pages
83-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC103535
Subset
IM
Databases
GENBANK
AJ003064, AJ010144
External Links
PubMed source DOI: 10.1128/JB.181.1.83-90.1999 PMC Full Text View journal details
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