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PMID: 9683501 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning, characterization, and transcriptional analysis of a gene encoding an alpha-crystallin-related, small heat shock protein from the thermophilic cyanobacterium Synechococcus vulcanus.

Journal of bacteriology ·Vol. 180 ·No. 15 ·1998-08-00 ·Pages 3997-4001

Roy SK, Nakamoto H

Abstract

hspA, a gene encoding a 16-kDa heat-induced protein from the thermophilic cyanobacterium Synechococcus vulcanus, has been cloned and sequenced. The deduced amino acid sequence of the gene product showed significant homology to sequences of the family of alpha-crystallin-related, small heat shock proteins. A monocistronic mRNA of hspA increased transiently in response to heat shock. The heat shock induction occurred at a vegetative promoter but without the CIRCE (controlling inverted repeat of chaperone expression) element.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/biosynthesis,chemistry,genetics Base Sequence Cloning, Molecular Crystallins/chemistry Cyanobacteria/genetics,metabolism DNA Primers Genes, Bacterial Heat-Shock Proteins/biosynthesis,chemistry,genetics Hot Temperature Molecular Sequence Data Promoter Regions, Genetic RNA, Messenger/biosynthesis,chemistry,drug effects Recombinant Proteins/biosynthesis,chemistry Rifampin/pharmacology Sequence Alignment Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Bacterial Proteins Crystallins DNA Primers Heat-Shock Proteins HspA protein, bacteria RNA, Messenger Recombinant Proteins Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roy S K
Department of Biochemistry and Molecular Biology, Saitama University, Urawa 338, Japan.
Nakamoto H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.15.3997-4001.1998
Published
1998-08-00
Pages
3997-4001
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107388
Subset
IM
Databases
GENBANK
AB002666
External Links
PubMed source DOI: 10.1128/JB.180.15.3997-4001.1998 PMC Full Text View journal details
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