Characterization of Streptomyces albus 18-kilodalton heat shock-responsive protein.
In Streptomyces albus during the heat shock response, a small heat shock protein of 18 kDa is dramatically induced. This protein was purified, and internal sequences revealed that S. albus HSP18 showed a marked homology with proteins belonging to the family of small heat shock proteins. The corresponding gene was isolated and sequenced. DNA sequence analysis confirmed that the hsp18 gene product is an analog of the 18-kDa antigen of Mycobacterium leprae. No hsp18 mRNA could be detected at 30 degrees C, but transcription of this gene was strongly induced following heat shock. The transcription initiation site was determined by nuclease S1 protection. A typical streptomycete vegetative promoter sequence was identified upstream from the initiation site. Disruption mutagenesis of hsp18 showed that HSP18 is not essential for growth in the 30 to 42 degrees C temperature range. However, HSP18 is involved in thermotolerance at extreme temperatures.
25 references, click to expand
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
-
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
-
DNA cloning in Streptomyces: resistance genes from antibiotic-producing species.Nature. 1980 Jul 31;286(5772):525-7 PMID: 6250070
-
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.Gene. 1985;33(1):103-19 PMID: 2985470
-
An ancient developmental induction: heat-shock proteins induced in sporulation and oogenesis.Science. 1986 Mar 7;231(4742):1154-7 PMID: 3511530
-
Asymmetric segregation of heat-shock proteins upon cell division in Caulobacter crescentus.J Mol Biol. 1987 Apr 20;194(4):653-62 PMID: 3309328
-
Acceleration of starvation- and glycerol-induced myxospore formation by prior heat shock in Myxococcus xanthus.J Bacteriol. 1988 Nov;170(11):5200-7 PMID: 3141380
-
A protein antigen of Mycobacterium leprae is related to a family of small heat shock proteins.J Bacteriol. 1988 Dec;170(12):5919-21 PMID: 3056923
-
Construction of a series of pSAM2-based integrative vectors for use in actinomycetes.Gene. 1990 Sep 28;94(1):53-9 PMID: 2227452
-
Nucleotide sequence analysis reveals linked N-acetyl hydrolase, thioesterase, transport, and regulatory genes encoded by the bialaphos biosynthetic gene cluster of Streptomyces hygroscopicus.J Bacteriol. 1991 Jul;173(14):4454-63 PMID: 2066341
-
A survey of the heat shock response in four Streptomyces species reveals two groEL-like genes and three groEL-like proteins in Streptomyces albus.J Bacteriol. 1991 Nov;173(22):7374-81 PMID: 1682303
-
Characterization of the groEL-like genes in Streptomyces albus.J Bacteriol. 1991 Nov;173(22):7382-6 PMID: 1682304
-
The 14,000-molecular-weight antigen of Mycobacterium tuberculosis is related to the alpha-crystallin family of low-molecular-weight heat shock proteins.J Bacteriol. 1992 Feb;174(4):1352-9 PMID: 1370952
-
Compilation and analysis of DNA sequences associated with apparent streptomycete promoters.Nucleic Acids Res. 1992 Mar 11;20(5):961-74 PMID: 1549509
-
Global changes in gene expression related to antibiotic synthesis in Streptomyces hygroscopicus.Mol Microbiol. 1992 Apr;6(8):969-80 PMID: 1584026
-
Homologs of Mycobacterium leprae 18-kilodalton and Mycobacterium tuberculosis 19-kilodalton antigens in other mycobacteria.Infect Immun. 1993 Apr;61(4):1509-15 PMID: 8454357
-
Sequence and molecular characterization of a DNA region encoding a small heat shock protein of Clostridium acetobutylicum.J Bacteriol. 1993 Jun;175(11):3394-400 PMID: 8501044
-
Heat shock proteins: molecular chaperones of protein biogenesis.Microbiol Rev. 1993 Jun;57(2):402-14 PMID: 8336673
-
Identification of nucleotide-binding regions in the chaperonin proteins GroEL and GroES.Nature. 1993 Nov 18;366(6452):279-82 PMID: 7901771
-
Use of new Escherichia coli/Streptomyces conjugative vectors to probe the functions of the two groEL-like genes of Streptomyces albus G by gene disruption.Gene. 1993 Nov 30;134(1):25-32 PMID: 7902308
-
CIRCE, a novel heat shock element involved in regulation of heat shock operon dnaK of Bacillus subtilis.J Bacteriol. 1994 Mar;176(5):1359-63 PMID: 8113175
-
The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins.Annu Rev Genet. 1993;27:437-96 PMID: 8122909
-
Assisting spontaneity: the role of Hsp90 and small Hsps as molecular chaperones.Trends Biochem Sci. 1994 May;19(5):205-11 PMID: 7914036
-
Post-transcriptional regulation of the groEL1 gene of Streptomyces albus.Mol Microbiol. 1994 May;12(3):423-32 PMID: 7915004
-
Streptomyces albus G mutants defective in the SalGI restriction-modification system.J Gen Microbiol. 1980 Feb;116(2):323-34 PMID: 6246193
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