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PMID: 9817748 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nuclear import and the evolution of a multifunctional RNA-binding protein.

The Journal of cell biology ·Vol. 143 ·No. 4 ·1998-11-16 ·Pages 887-99

Rosenblum JS, Pemberton LF, Bonifaci N, Blobel G

Abstract

La (SS-B) is a highly expressed protein that is able to bind 3'-oligouridylate and other common RNA sequence/structural motifs. By virtue of these interactions, La is present in a myriad of nuclear and cytoplasmic ribonucleoprotein complexes in vivo where it may function as an RNA-folding protein or RNA chaperone. We have recently characterized the nuclear import pathway of the S. cerevisiae La, Lhp1p. The soluble transport factor, or karyopherin, that mediates the import of Lhp1p is Kap108p/Sxm1p. We have now determined a 113-amino acid domain of Lhp1p that is brought to the nucleus by Kap108p. Unexpectedly, this domain does not coincide with the previously identified nuclear localization signal of human La. Furthermore, when expressed in Saccharomyces cerevisiae, the nuclear localization of Schizosaccharomyces pombe, Drosophila, and human La proteins are independent of Kap108p. We have been able to reconstitute the nuclear import of human La into permeabilized HeLa cells using the recombinant human factors karyopherin alpha2, karyopherin beta1, Ran, and p10. As such, the yeast and human La proteins are imported using different sequence motifs and dissimilar karyopherins. Our results are consistent with an intermingling of the nuclear import and evolution of La.

MeSH Terms
Autoantigens/chemistry,genetics,metabolism Evolution, Molecular Fungal Proteins/metabolism Gene Expression Regulation, Fungal Green Fluorescent Proteins Humans Indicators and Reagents Luminescent Proteins Molecular Sequence Data Nuclear Localization Signals/physiology Nuclear Proteins/metabolism Phylogeny Protein Structure, Tertiary RNA, Fungal/biosynthesis RNA-Binding Proteins/physiology Ribonucleoproteins/chemistry,genetics,metabolism Saccharomyces cerevisiae/chemistry,genetics,metabolism Sequence Homology, Amino Acid Transcription Factors/chemistry,genetics,metabolism alpha Karyopherins
Chemicals
Autoantigens Fungal Proteins Indicators and Reagents Luminescent Proteins Nuclear Localization Signals Nuclear Proteins RNA, Fungal RNA-Binding Proteins Ribonucleoproteins SS-B antigen Transcription Factors alpha Karyopherins Green Fluorescent Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rosenblum J S
Laboratory of Cell Biology, Howard Hughes Medical Institute and Rockefeller University, New York, New York 10021, USA.
Pemberton L F
Bonifaci N
Blobel G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.143.4.887
Published
1998-11-16
Pages
887-99
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132966
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.143.4.887 PMC Full Text View journal details
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