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PMID: 9412460 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A distinct and parallel pathway for the nuclear import of an mRNA-binding protein.

The Journal of cell biology ·Vol. 139 ·No. 7 ·1997-12-29 ·Pages 1645-53

Pemberton LF, Rosenblum JS, Blobel G

Abstract

Three independent pathways of nuclear import have so far been identified in yeast, each mediated by cognate nuclear transport factors, or karyopherins. Here we have characterized a new pathway to the nucleus, mediated by Mtr10p, a protein first identified in a screen for strains defective in polyadenylated RNA export. Mtr10p is shown to be responsible for the nuclear import of the shuttling mRNA-binding protein Npl3p. A complex of Mtr10p and Npl3p was detected in cytosol, and deletion of Mtr10p was shown to lead to the mislocalization of nuclear Npl3p to the cytoplasm, correlating with a block in import. Mtr10p bound peptide repeat-containing nucleoporins and Ran, suggesting that this import pathway involves a docking step at the nuclear pore complex and is Ran dependent. This pathway of Npl3p import is distinct and does not appear to overlap with another known import pathway for an mRNA-binding protein. Thus, at least two parallel pathways function in the import of mRNA-binding proteins, suggesting the need for the coordination of these pathways.

MeSH Terms
Amino Acid Sequence Biological Transport Cell Nucleus/metabolism Cytoplasm/metabolism Cytosol/metabolism Fungal Proteins/metabolism GTP-Binding Proteins/metabolism Molecular Sequence Data Monomeric GTP-Binding Proteins Nuclear Proteins/chemistry,metabolism Nucleocytoplasmic Transport Proteins Peptides/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/chemistry,metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction beta Karyopherins
Chemicals
Fungal Proteins GSP1 protein, S cerevisiae MTR10 protein, S cerevisiae NPL3 protein, S cerevisiae Nuclear Proteins Nucleocytoplasmic Transport Proteins Peptides RNA, Messenger RNA-Binding Proteins Saccharomyces cerevisiae Proteins beta Karyopherins GTP-Binding Proteins Monomeric GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pemberton L F
Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York 10021, USA.
Rosenblum J S
Blobel G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.139.7.1645
Published
1997-12-29
Pages
1645-53
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132632
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.139.7.1645 PMC Full Text View journal details
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