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PMID: 7730395 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A nuclear localization domain in the hnRNP A1 protein.

The Journal of cell biology ·Vol. 129 ·No. 3 ·1995-05-00 ·Pages 551-60

Siomi H, Dreyfuss G

Abstract

The heterogeneous nuclear RNP (hnRNP) A1 protein is one of the major pre-mRNA/mRNA binding proteins in eukaryotic cells and one of the most abundant proteins in the nucleus. It is localized to the nucleoplasm and it also shuttles between the nucleus and the cytoplasm. The amino acid sequence of A1 contains two RNP motif RNA-binding domains (RBDs) at the amino terminus and a glycine-rich domain at the carboxyl terminus. This configuration, designated 2x RBD-Gly, is representative of perhaps the largest family of hnRNP proteins. Unlike most nuclear proteins characterized so far, A1 (and most 2x RBD-Gly proteins) does not contain a recognizable nuclear localization signal (NLS). We have found that a segment of ca. 40 amino acids near the carboxyl end of the protein (designated M9) is necessary and sufficient for nuclear localization; attaching this segment to the bacterial protein beta-galactosidase or to pyruvate kinase completely localized these otherwise cytoplasmic proteins to the nucleus. The RBDs and another RNA binding motif found in the glycine-rich domain, the RGG box, are not required for A1 nuclear localization. M9 is a novel type of nuclear localization domain as it does not contain sequences similar to classical basic-type NLS. Interestingly, sequences similar to M9 are found in other nuclear RNA-binding proteins including hnRNP A2.

MeSH Terms
Amino Acid Sequence Animals Biological Transport Cell Compartmentation Cell Nucleus/metabolism Cells, Cultured Fluorescent Antibody Technique Haplorhini Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Kidney/cytology Molecular Sequence Data Poly(A)-Binding Proteins Pyruvate Kinase/genetics,metabolism RNA-Binding Proteins/genetics,metabolism Recombinant Proteins/metabolism Ribonucleoproteins/genetics,metabolism Structure-Activity Relationship Transfection beta-Galactosidase/genetics,metabolism
Chemicals
Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Poly(A)-Binding Proteins RNA-Binding Proteins Recombinant Proteins Ribonucleoproteins hnRNP A2 Pyruvate Kinase beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Siomi H
Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104-6148, USA.
Dreyfuss G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.129.3.551
Published
1995-05-00
Pages
551-60
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120450
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.129.3.551 PMC Full Text View journal details
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