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PMID: 8041722 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The A1 and A1B proteins of heterogeneous nuclear ribonucleoparticles modulate 5' splice site selection in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 15 ·1994-07-19 ·Pages 6924-8

Yang X, Bani MR, Lu SJ, Rowan S, Ben-David Y, Chabot B

Abstract

Recent in vitro results suggest that the heterogeneous nuclear ribonucleoparticle (hnRNP) A1 protein modulates alternative splicing by favoring distal 5' splice site (5'SS) selection and exon skipping. We used a mouse erythroleukemia (MEL) cell line (CB3C7) deficient in the expression of hnRNP A1 to test whether variations in hnRNP A1 and AlB protein levels affected alternative splicing in vivo. In contrast to A1-expressing MEL cell lines, CB3C7 cells preferentially selected the proximal 13S and 12S 5'SS on the adenovirus E1A pre-mRNA. Transiently expressing the A1 or A1B cDNA in CB3C7 cells shifted 5'SS selection toward the more distal 9S donor site. A1 protein synthesis was required for this effect since the expression of a mutated A1 cDNA did not affect 5'SS selection. These results demonstrate that in vivo variations in hnRNP A1 protein levels can influence 5'SS selection.

MeSH Terms
Alternative Splicing Animals Base Sequence DNA Exons Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Mice Molecular Sequence Data Mutagenesis, Insertional RNA Precursors/metabolism Ribonucleoproteins/metabolism Transfection Tumor Cells, Cultured
Chemicals
Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins RNA Precursors Ribonucleoproteins DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yang X
Département de Microbiologie, Faculté de Médecine, Université de Sherbrooke, PQ Canada.
Bani M R
Lu S J
Rowan S
Ben-David Y
Chabot B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.15.6924
Published
1994-07-19
Pages
6924-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44310
Subset
IM
Databases
GENBANK
X78885
External Links
PubMed source DOI: 10.1073/pnas.91.15.6924 PMC Full Text View journal details
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