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PMID: 8227139 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of the yeast nuclear pore complex.

The Journal of cell biology ·Vol. 123 ·No. 4 ·1993-11-00 ·Pages 771-83

Rout MP, Blobel G

Abstract

Nuclear pore complexes (NPCs) have been isolated from the yeast Saccharomyces. Negative stain electron microscopy of the isolated NPCs and subsequent image reconstruction revealed the octagonal symmetry and many of the ultrastructural features characteristic of vertebrate NPCs. The overall dimensions of the yeast NPC, both in its isolated form as well as in situ, are smaller than its vertebrate counterpart. However, the diameter of the central structures are similar. The isolated yeast NPC has a sedimentation coefficient of approximately 310 S and an M(r) of approximately 66 MD. It retains all but one of the eight known NPC proteins. In addition it contains as many as 80 uncharacterized proteins that are candidate NPC proteins.

MeSH Terms
Cell Fractionation Chromatography, High Pressure Liquid Chromatography, Ion Exchange Fungal Proteins/isolation & purification Microscopy, Electron Nuclear Envelope/chemistry,ultrastructure Saccharomyces cerevisiae/ultrastructure
Chemicals
Fungal Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rout M P
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.
Blobel G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.123.4.771
Published
1993-11-00
Pages
771-83
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200146
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.123.4.771 PMC Full Text View journal details
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