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PMID: 9606195 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TTF-I determines the chromatin architecture of the active rDNA promoter.

The EMBO journal ·Vol. 17 ·No. 11 ·1998-06-01 ·Pages 3135-45

Längst G, Becker PB, Grummt I

Abstract

Transcription of ribosomal genes assembled into chromatin requires binding of the transcription termination factor TTF-I to the promoter-proximal terminator T0. To analyze the mechanism of TTF-I-mediated transcriptional activation, we have used mutant templates with altered sequence, polarity and distance of T0 with respect to the transcription start site. Transcription activation by TTF-I is chromatin specific and requires the precise positioning of the terminator relative to the promoter. Whereas termination by TTF-I depends on the correct orientation of a terminator, TTF-I-mediated transcriptional activation is orientation independent. TTF-I can bind to nucleosomal DNA in the absence of enzymatic activities that destabilize nucleosome structure. Chromatin-bound TTF-I synergizes with ATP-dependent cofactors present in extracts of Drosophila embryos and mouse cells to position a nucleosome over the rDNA promoter and the transcription start site. Nucleosome positioning correlates tightly with the activation of rDNA transcription. We suggest that transcriptional activation by TTF-I is a stepwise process involving the creation of a defined promoter architecture and that the positioning of a nucleosome is compatible with, if not a prerequisite for, transcription initiation from rDNA chromatin.

MeSH Terms
Adenosine Triphosphate/physiology Animals Binding Sites/genetics Cell-Free System Chromatin/genetics,metabolism Codon, Terminator/physiology DNA/metabolism DNA Polymerase I/genetics DNA, Ribosomal/genetics,metabolism DNA-Binding Proteins/genetics,metabolism,physiology Drosophila/embryology,genetics,metabolism HeLa Cells Humans Mice Nucleosomes/genetics,metabolism Peptide Chain Termination, Translational/genetics Promoter Regions, Genetic Protein Binding/genetics RNA Polymerase I/genetics Transcription Factors Transcriptional Activation
Chemicals
Chromatin Codon, Terminator DNA, Ribosomal DNA-Binding Proteins Nucleosomes TTF1 protein, human Transcription Factors Ttf1 protein, mouse Adenosine Triphosphate DNA RNA Polymerase I DNA Polymerase I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Längst G
German Cancer Research Center, Division of Molecular Biology of the Cell II, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
Becker P B
Grummt I
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.11.3135
Published
1998-06-01
Pages
3135-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170652
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/17.11.3135 PMC Full Text View journal details
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