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PMID: 7774579 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Energy-dependent chromatin accessibility and nucleosome mobility in a cell-free system.

The EMBO journal ·Vol. 14 ·No. 10 ·1995-05-15 ·Pages 2209-16

Varga-Weisz PD, Blank TA, Becker PB

Abstract

Chromatin structure must be flexible to allow the binding of regulatory proteins and to accommodate different levels of gene activity. Chromatin assembled in a cell-free system derived from Drosophila embryos contains an activity that hydrolyses ATP to render entire nucleosome arrays mobile. Nucleosome movements, most likely their sliding, occurred even in the presence of the linker histone H1. The dynamic state of chromatin in the presence of the activity and ATP globally increased the accessibility of nucleosomal DNA to incoming proteins. This unprecedented demonstration of energy-dependent nucleosome mobility identifies a new principle which is likely to be fundamental to the mechanism of chromatin remodelling and the binding of regulatory proteins.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Cell-Free System DNA/metabolism DNA-Binding Proteins/metabolism Deoxyribonucleases, Type II Site-Specific/metabolism Drosophila/embryology,genetics Drosophila Proteins Energy Metabolism HSP70 Heat-Shock Proteins/genetics Histones/metabolism Homeodomain Proteins Models, Genetic Movement/physiology Nucleosomes/physiology Promoter Regions, Genetic/genetics Transcription Factors/metabolism
Chemicals
DNA-Binding Proteins Drosophila Proteins HSP70 Heat-Shock Proteins Histones Homeodomain Proteins Nucleosomes Transcription Factors Trl protein, Drosophila Adenosine Triphosphate DNA Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Varga-Weisz P D
European Molecular Biology Laboratory, Heidelberg, Germany.
Blank T A
Becker P B
References (35)
35 references, click to expand
  1. Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation.
    Cell. 1990 Nov 30;63(5):1085-97 PMID: 2257625
  2. Chromatin remodeling by GAGA factor and heat shock factor at the hypersensitive Drosophila hsp26 promoter in vitro.
    EMBO J. 1995 Apr 18;14(8):1727-36 PMID: 7737124
  3. Facilitated binding of GAL4 and heat shock factor to nucleosomal templates: differential function of DNA-binding domains.
    Genes Dev. 1991 Jul;5(7):1285-98 PMID: 2065977
  4. Mobility of positioned nucleosomes on 5 S rDNA.
    J Mol Biol. 1991 Jul 5;220(1):101-10 PMID: 2067009
  5. Glucocorticoids are required for establishment and maintenance of an alteration in chromatin structure: induction leads to a reversible disruption of nucleosomes over an enhancer.
    EMBO J. 1991 Sep;10(9):2569-76 PMID: 1678348
  6. brahma: a regulator of Drosophila homeotic genes structurally related to the yeast transcriptional activator SNF2/SWI2.
    Cell. 1992 Feb 7;68(3):561-72 PMID: 1346755
  7. Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription.
    Cell. 1992 Feb 7;68(3):573-83 PMID: 1339306
  8. Transcription factor loading on the MMTV promoter: a bimodal mechanism for promoter activation.
    Science. 1992 Mar 20;255(5051):1573-6 PMID: 1347958
  9. Mobility of histones on the chromosome of simian virus 40.
    Cell. 1978 Nov;15(3):955-67 PMID: 31980
  10. Reassociation of histone H1 with nucleosomes.
    J Biol Chem. 1979 Nov 25;254(22):11751-60 PMID: 500671
  11. Four Drosophila heat shock genes at 67B: characterization of recombinant plasmids.
    Nucleic Acids Res. 1980 Oct 10;8(19):4441-57 PMID: 6253919
  12. Regulation of the higher-order structure of chromatin by histones H1 and H5.
    J Cell Biol. 1981 Aug;90(2):279-88 PMID: 7287811
  13. Histones H1 and H5: one or two molecules per nucleosome?
    Nucleic Acids Res. 1981 Nov 25;9(22):5883-94 PMID: 7312631
  14. Mechanism of antitumor drug action: poisoning of mammalian DNA topoisomerase II on DNA by 4'-(9-acridinylamino)-methanesulfon-m-anisidide.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1361-5 PMID: 6324188
  15. Inhibition of the DNA catenation activity of type II topoisomerase by VP16-213 and VM26.
    Biochem Biophys Res Commun. 1984 Jul 18;122(1):165-70 PMID: 6331440
  16. Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements.
    EMBO J. 1986 Oct;5(10):2689-96 PMID: 3536481
  17. Cell-free system for assembly of transcriptionally repressed chromatin from Drosophila embryos.
    Mol Cell Biol. 1992 May;12(5):2241-9 PMID: 1569951
  18. The reprogramming of transcriptional competence.
    Cell. 1992 May 15;69(4):573-5 PMID: 1316804
  19. Chromatin dynamics and the modulation of genetic activity.
    Trends Biochem Sci. 1992 May;17(5):187-91 PMID: 1595128
  20. Mobile nucleosomes--a general behavior.
    EMBO J. 1992 Aug;11(8):2951-9 PMID: 1639066
  21. Nucleosome displacement in transcription.
    Cell. 1993 Feb 12;72(3):305-8 PMID: 8431942
  22. The yeast SNF2/SWI2 protein has DNA-stimulated ATPase activity required for transcriptional activation.
    Genes Dev. 1993 Apr;7(4):583-91 PMID: 8458575
  23. (CT)n (GA)n repeats and heat shock elements have distinct roles in chromatin structure and transcriptional activation of the Drosophila hsp26 gene.
    Mol Cell Biol. 1993 May;13(5):2802-14 PMID: 8474442
  24. Histones, nucleosomes and transcription.
    Curr Opin Genet Dev. 1993 Apr;3(2):219-25 PMID: 8389216
  25. Transcriptional repression by nucleosomes but not H1 in reconstituted preblastoderm Drosophila chromatin.
    EMBO J. 1994 Jan 15;13(2):373-9 PMID: 8313882
  26. ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor.
    Nature. 1994 Feb 10;367(6463):525-32 PMID: 8107823
  27. Transcription: in tune with the histones.
    Cell. 1994 Apr 8;77(1):13-6 PMID: 8156588
  28. Architectural variations of inducible eukaryotic promoters: preset and remodeling chromatin structures.
    Bioessays. 1994 Mar;16(3):165-70 PMID: 8166669
  29. Nucleosome disruption and enhancement of activator binding by a human SW1/SNF complex.
    Nature. 1994 Aug 11;370(6489):477-81 PMID: 8047169
  30. Facilitated binding of TATA-binding protein to nucleosomal DNA.
    Nature. 1994 Aug 11;370(6489):481-5 PMID: 8047170
  31. The establishment of active promoters in chromatin.
    Bioessays. 1994 Aug;16(8):541-7 PMID: 8086003
  32. The Trithorax-like gene encodes the Drosophila GAGA factor.
    Nature. 1994 Oct 27;371(6500):806-8 PMID: 7935842
  33. Linker histones H1 and H5 prevent the mobility of positioned nucleosomes.
    Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10275-9 PMID: 7937940
  34. Chromatin assembly extracts from Drosophila embryos.
    Methods Cell Biol. 1994;44:207-23 PMID: 7707953
  35. The interaction of histone H5 and its globular domain with core particles, depleted chromatosomes, polynucleosomes, and a DNA decamer.
    J Biol Chem. 1991 Jan 25;266(3):1502-8 PMID: 1988433
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-05-15
Pages
2209-16
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398327
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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