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PMID: 9528795 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The tomato Hsf system: HsfA2 needs interaction with HsfA1 for efficient nuclear import and may be localized in cytoplasmic heat stress granules.

Molecular and cellular biology ·Vol. 18 ·No. 4 ·1998-04-00 ·Pages 2240-51

Scharf KD, Heider H, Höhfeld I, Lyck R, Schmidt E, Nover L

Abstract

In heat-stressed (HS) tomato (Lycopersicon peruvianum) cell cultures, the constitutively expressed HS transcription factor HsfA1 is complemented by two HS-inducible forms, HsfA2 and HsfB1. Because of its stability, HsfA2 accumulates to fairly high levels in the course of a prolonged HS and recovery regimen. Using immunofluorescence and cell fractionation experiments, we identified three states of HsfA2: (i) a soluble, cytoplasmic form in preinduced cultures maintained at 25 degrees C, (ii) a salt-resistant, nuclear form found in HS cells, and (iii) a stored form of HsfA2 in cytoplasmic HS granules. The efficient nuclear transport of HsfA2 evidently requires interaction with HsfA1. When expressed in tobacco protoplasts by use of a transient-expression system, HsfA2 is mainly retained in the cytoplasm unless it is coexpressed with HsfA1. The essential parts for the interaction and nuclear cotransport of the two Hsfs are the homologous oligomerization domain (HR-A/B region of the A-type Hsfs) and functional nuclear localization signal motifs of both partners. Direct physical interaction of the two Hsfs with formation of relatively stabile hetero-oligomers was shown by a two-hybrid test in Saccharomyces cerevisiae as well as by coimmunoprecipitation using tomato and tobacco whole-cell lysates.

MeSH Terms
Amino Acid Sequence Biological Transport Cell Nucleus/metabolism Cells, Cultured Cytoplasmic Granules/metabolism DNA-Binding Proteins/biosynthesis,metabolism Heat Shock Transcription Factors Heat-Shock Proteins/metabolism Lycopersicon esculentum/metabolism Molecular Sequence Data Plant Proteins Plants, Toxic Precipitin Tests Protoplasts Tobacco/metabolism Transcription Factors/biosynthesis,metabolism
Chemicals
DNA-Binding Proteins Heat Shock Transcription Factors Heat-Shock Proteins Plant Proteins Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Scharf K D
Department of Molecular Cell Biology, Goethe University Frankfurt, Frankfurt/Main, Germany.
Heider H
Höhfeld I
Lyck R
Schmidt E
Nover L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.4.2240
Published
1998-04-00
Pages
2240-51
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC121470
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.18.4.2240 PMC Full Text View journal details
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