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PMID: 2148291 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Three tomato genes code for heat stress transcription factors with a region of remarkable homology to the DNA-binding domain of the yeast HSF.

The EMBO journal ·Vol. 9 ·No. 13 ·1990-12-00 ·Pages 4495-501

Scharf KD, Rose S, Zott W, Schöffl F, Nover L, Schöff F

Abstract

Heat stress (hs) treatment of cell cultures of Lycopersicon peruvianum (Lp, tomato) results in activation of preformed transcription factor(s) (HSF) binding to the heat stress consensus element (HSE). Using appropriate synthetic HSE oligonucleotides, three types of clones with potential HSE binding domains were isolated from a tomato lambda gt11 expression library by DNA-ligand screening. One of the potential HSF genes is constitutively expressed, the other two are hs-induced. Sequence comparison defines a single domain of approximately 90 amino acid residues common to all three genes and to the HSE--binding domain of the yeast HSF. The domain is flanked by proline residues and characterized by two long overlapping repeats. We speculate that the derived consensus sequence is also representative for other eukaryotic HSF and that the existence of several different HSF is not unique to plants.

Related Genes
HSF
MeSH Terms
Amino Acid Sequence Bacteriophage lambda/genetics Base Sequence Cloning, Molecular Consensus Sequence DNA/chemistry,metabolism Genomic Library Heat-Shock Proteins/genetics Hot Temperature Molecular Sequence Data Plants/genetics RNA, Messenger/metabolism Sequence Homology, Nucleic Acid Transcription Factors/genetics Yeasts/genetics
Chemicals
Heat-Shock Proteins RNA, Messenger Transcription Factors DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Scharf K D
Department of Stress Research, Institute of Plant Biochemistry, Halle, FRG.
Rose S
Zott W
Schöffl F
Nover L
Schöff F
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38 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-12-00
Pages
4495-501
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552242
Subset
IM
Databases
GENBANK
X55347
Corrections
ErratumIn
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External Links
PubMed source PMC Full Text View journal details
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