Abstract
The heat shock response is transcriptionally regulated by an evolutionarily conserved protein termed heat shock factor (HSF). We report the purification to homogeneity and the partial peptide sequence of HSF from HeLa cells. The peptide sequence was used to isolate a human cDNA with a predicted open reading frame that has homology to the DNA binding domains of both Saccharomyces cerevisiae and Drosophila HSFs. The cDNA directs the synthesis of a protein that binds to the heat shock element with specificity identical to HeLa HSF and stimulates transcription from a heat shock promoter. The expressed protein cross-reacts with anti-HSF antibodies. Surprisingly, however, this cDNA does not encode all of the peptides obtained from purified HeLa HSF. These peptides are encoded by a distinct human cDNA, HSF1, described by Rabindran et al. [Rabindran, S. K., Giorgi, G., Clos, J. & Wu, C. (1991) Proc. Natl. Acad. Sci. USA 88, 6906-6910.] It therefore appears that there is a human heat shock factor gene family and that at least two separate but related HSF proteins regulate the stress response in humans.
MeSH Terms
Amino Acid Sequence
Animals
Base Sequence
Biological Evolution
Cloning, Molecular
DNA, Neoplasm/genetics,isolation & purification
Drosophila/genetics
Genes
HeLa Cells/physiology
Heat-Shock Proteins/genetics,isolation & purification
Humans
Molecular Sequence Data
Promoter Regions, Genetic
Protein Biosynthesis
Recombinant Proteins/isolation & purification
Saccharomyces cerevisiae/genetics
Sequence Homology, Nucleic Acid
Transcription Factors/genetics,isolation & purification
Transcription, Genetic
Chemicals
DNA, Neoplasm
Heat-Shock Proteins
Recombinant Proteins
Transcription Factors
HSF2 protein, human
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schuetz T J
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Gallo G J
Sheldon L
Tempst P
Kingston R E
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