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PMID: 2203532 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning of the p50 DNA binding subunit of NF-kappa B: homology to rel and dorsal.

Cell ·Vol. 62 ·No. 5 ·1990-09-07 ·Pages 1019-29

Ghosh S, Gifford AM, Riviere LR, Tempst P, Nolan GP, Baltimore D

Abstract

The DNA binding subunit of the transcription factor NF-kappa B, p50, has been cloned. p50 appears to be synthesized as a larger protein that is then processed to its functional size. Sequence analysis reveals remarkable homology for over 300 amino acids at the amino-terminal end to the oncogene v-rel, its cellular homolog c-rel, and the Drosophila maternal effect gene dorsal. This establishes NF-kappa B as a member of the rel family of proteins, all of which display nuclear-cytosolic translocation. Protein sequence from the p65 polypeptide has established that it is not encoded in the same mRNA as p50. However, p65 appears homologous to c-rel, suggesting that c-rel may form heterodimers with p50 and rel may include a homodimerization motif.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cloning, Molecular/methods DNA/genetics,isolation & purification DNA-Binding Proteins/genetics,isolation & purification Drosophila/genetics Female Genes Humans Lung/metabolism Macromolecular Substances Mice Molecular Sequence Data NF-kappa B Oncogene Proteins v-rel Peptide Fragments/isolation & purification Protein Biosynthesis Protein-Tyrosine Kinases/genetics Rabbits Recombinant Proteins/isolation & purification Retroviridae Proteins, Oncogenic/genetics Sequence Homology, Nucleic Acid Transcription Factors/genetics,isolation & purification Transcription, Genetic Trypsin

Chemicals

DNA-Binding Proteins Macromolecular Substances NF-kappa B Oncogene Proteins v-rel Peptide Fragments Recombinant Proteins Retroviridae Proteins, Oncogenic Transcription Factors DNA Protein-Tyrosine Kinases Trypsin

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Ghosh S

Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142.

Gifford A M

Riviere L R

Tempst P

Nolan G P

Baltimore D

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/0092-8674(90)90276-k

Published

1990-09-07

Pages

1019-29

Language

English

Region

United States

NLM ID

0413066

Subset

Grants

NIGMS NIH HHS · GM39458 · United States

Databases

GENBANK

M37732, M57999

External Links

PubMed source DOI: 10.1016/0092-8674(90)90276-k View journal details

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Cloning of the p50 DNA binding subunit of NF-kappa B: homology to rel and dorsal.

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