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PMID: 2437578 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 10 ·1987-05-00 ·Pages 3365-9

Seed B, Aruffo A

Abstract

A cDNA encoding the CD2 antigen has been isolated by a highly efficient technique based on transient expression in COS cells and adherence of cells expressing surface antigen to antibody-coated dishes. COS cells expressing a CD2 cDNA isolated by this method readily formed rosettes with sheep as well as human and other mammalian erythrocytes. Pretreatment of transfected COS cells with anti-CD2 antibody, or pretreatment of human erythrocytes with anti-LFA-3 antibody, abolished rosette formation.

MeSH Terms
Amino Acid Sequence Animals Antigens, Differentiation, T-Lymphocyte Antigens, Surface/genetics Base Sequence Cell Line Cloning, Molecular DNA/metabolism Epitopes/analysis Erythrocytes/immunology Genetic Vectors Humans T-Lymphocytes/immunology
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Surface Epitopes DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Seed B
Aruffo A
References (36)
36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.10.3365
Published
1987-05-00
Pages
3365-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304871
Subset
IM
Databases
GENBANK
M16445
External Links
PubMed source DOI: 10.1073/pnas.84.10.3365 PMC Full Text View journal details
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