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PMID: 7948881 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Arabidopsis heat shock factor: isolation and characterization of the gene and the recombinant protein.

Plant molecular biology ·Vol. 26 ·No. 1 ·1994-10-00 ·Pages 353-62

Hübel A, Schöffl F

Abstract

We have isolated the Arabidopsis heat shock factor gene Athsf1 as genomic and corresponding cDNA sequences via cross-hybridization with tomato clones. Sequence analysis indicates only a partial homology with the HSFs from tomato and other organisms which is confined to the DNA-binding and the oligomerization domains. The gene is constitutively expressed but the level of mRNA for Athsf1 increases two-fold upon heat shock. However, the putative promoter region lacks the canonical heat shock elements. After expression in Escherichia coli the recombinant Athsf1 protein binds specifically to a synthetic oligonucleotide containing five heat shock elements. The native size of recombinant ATHSF1 in vitro is consistent with a trimer as demonstrated by chemical cross-linking and pore exclusion limit analysis.

Related Genes
Athsf1
MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Arabidopsis Proteins Base Sequence Cloning, Molecular DNA-Binding Proteins Escherichia coli/genetics Gene Expression Genes, Plant/genetics Heat Shock Transcription Factors Heat-Shock Proteins/chemistry,genetics,metabolism Hot Temperature Lycopersicon esculentum/genetics Molecular Sequence Data Molecular Weight Plant Proteins/chemistry,genetics,metabolism Polyribonucleotides/chemical synthesis,metabolism Protein Conformation RNA, Messenger/analysis RNA, Plant/analysis Recombinant Proteins/chemistry,metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription Factors
Chemicals
Arabidopsis Proteins DNA-Binding Proteins Heat Shock Transcription Factors Heat-Shock Proteins Plant Proteins Polyribonucleotides RNA, Messenger RNA, Plant Recombinant Proteins Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hübel A
Universität Tübingen, Biologisches Institut, Germany.
Schöffl F
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34 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00039545
Published
1994-10-00
Pages
353-62
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
X76167
External Links
PubMed source DOI: 10.1007/BF00039545 View journal details
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