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PMID: 9524124 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells.

The EMBO journal ·Vol. 17 ·No. 7 ·1998-04-01 ·Pages 2019-32

Okuda A, Fukushima A, Nishimoto M, Orimo A, Yamagishi T, Nabeshima Y, Kuro-o M, Nabeshima Yi, Boon K, Keaveney M, Stunnenberg HG, Muramatsu M

Abstract

We have obtained a novel transcriptional cofactor, termed undifferentiated embryonic cell transcription factor 1 (UTF1), from F9 embryonic carcinoma (EC) cells. This protein is expressed in EC and embryonic stem cells, as well as in germ line tissues, but could not be detected in any of the other adult mouse tissues tested. Furthermore, when EC cells are induced to differentiate, UTF1 expression is rapidly extinguished. In normal mouse embryos, UTF1 mRNA is present in the inner cell mass, the primitive ectoderm and the extra-embryonic tissues. During the primitive streak stage, the induction of mesodermal cells is accompanied by the down-regulation of UTF1 in the primitive ectoderm. However, its expression is maintained for up to 13.5 days post-coitum in the extra-embryonic tissue. Functionally, UTF1 boosts the level of transcription of the adenovirus E2A promoter. However, unlike the pluripotent cell-specific E1A-like activity, which requires the E2F sites of the E2A promoter for increased transcriptional activation, UTF1-mediated activation is dependent on the upstream ATF site of this promoter. This result indicates that UTF1 is not a major component of the E1A-like activity present in pluripotent embryonic cells. Further analyses revealed that UTF1 interacts not only with the activation domain of ATF-2, but also with the TFIID complex in vivo. Thus, UTF1 displays many of the hallmark characteristics expected for a tissue-specific transcriptional coactivator that works in early embryogenesis.

MeSH Terms
Activating Transcription Factor 2 Adenovirus E2 Proteins/genetics Amino Acid Sequence Animals Base Sequence CHO Cells Cell Differentiation Chromosomal Proteins, Non-Histone Cloning, Molecular Cricetinae Cyclic AMP Response Element-Binding Protein/metabolism Embryonal Carcinoma Stem Cells Female Gene Expression Regulation, Developmental/physiology Male Mice Molecular Sequence Data Neoplastic Stem Cells/chemistry,cytology Organ Specificity Promoter Regions, Genetic/genetics RNA, Messenger/analysis Sequence Analysis, DNA Stem Cells/chemistry Trans-Activators/genetics,metabolism Transcription Factor TFIID Transcription Factors/metabolism Transcription Factors, TFII/metabolism
Chemicals
Activating Transcription Factor 2 Adenovirus E2 Proteins Atf2 protein, mouse Chromosomal Proteins, Non-Histone Cyclic AMP Response Element-Binding Protein RNA, Messenger Trans-Activators Transcription Factor TFIID Transcription Factors Transcription Factors, TFII Utf1 protein, mouse
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Okuda A
Department of Biochemistry, Saitama Medical School, 38 Morohongo, Moroyama, Iruma-gun, Saitama 350-0495, Japan.
Fukushima A
Nishimoto M
Orimo A
Yamagishi T
Nabeshima Y
Kuro-o M
Nabeshima Y i
Boon K
Keaveney M
Stunnenberg H G
Muramatsu M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.7.2019
Published
1998-04-01
Pages
2019-32
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170547
Subset
IM
Databases
GENBANK
D31647
External Links
PubMed source DOI: 10.1093/emboj/17.7.2019 PMC Full Text View journal details
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