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PMID: 2323334 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional cooperativity between two TPA responsive elements in undifferentiated F9 embryonic stem cells.

The EMBO journal ·Vol. 9 ·No. 4 ·1990-04-00 ·Pages 1131-5

Okuda A, Imagawa M, Sakai M, Muramatsu M

Abstract

We have recently identified an enhancer, termed GPEI, in the 5'-flanking region of the rat glutathione transferase P gene, that is composed of two imperfect TPA (phorbol 12-O-tetradecanoate 13-acetate) responsive elements (TREs). Unlike other TRE-containing enhancers, GPEI exhibits a strong transcriptional enhancing activity in F9 embryonic stem cells. Mutational analyses have revealed that the high activity of GPEI is mediated by two imperfect TREs. Each TRE-like sequence has no activity by itself but acts synergistically to form a strong enhancer which is active even in the very low level of AP-1 activity in F9 cells. Furthermore, we show that synthetic DNAs containing two perfect TREs in certain arrangements have strong transcriptional enhancing activities in F9 cells and the activity is greatly influenced by the relative orientation and the distance of two TREs.

MeSH Terms
Animals Base Sequence Cell Differentiation Cell Line DNA Probes Enhancer Elements, Genetic/drug effects Glutathione Transferase/genetics HeLa Cells/metabolism Humans Molecular Sequence Data Oligonucleotide Probes Plasmids Rats Teratoma Tetradecanoylphorbol Acetate/pharmacology Transfection
Chemicals
DNA Probes Oligonucleotide Probes Glutathione Transferase Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Okuda A
Department of Biochemistry, University of Tokyo Faculty of Medicine, Japan.
Imagawa M
Sakai M
Muramatsu M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-04-00
Pages
1131-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551788
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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