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PMID: 9449286 Published · ppublish English Journal Article

Cloning and nucleotide sequence of the DNA gyrase gyrA gene from Serratia marcescens and characterization of mutations in gyrA of quinolone-resistant clinical isolates.

Antimicrobial agents and chemotherapy ·Vol. 42 ·No. 1 ·1998-01-00 ·Pages 190-3

Kim JH, Cho EH, Kim KS, Kim HY, Kim YM

Abstract

The sequence of the DNA gyrase gyrA gene of Serratia marcescens ATCC 14756 was determined. An open reading frame of 2,640 nucleotides coding for a polypeptide with a calculated molecular mass of 97,460 was found, and its sequence complemented the sequence of an Escherichia coli gyrA temperature-sensitive mutation. Analysis of the PCR products of the quinolone resistance-determining regions of gyrA genes from six quinolone-resistant clinical isolates revealed a single amino acid substitution, Ser-83 to Arg or Asp-87 to Tyr, in all six mutants, suggesting that a mutational alteration in gyrA is a common mechanism of quinolone resistance in S. marcescens.

MeSH Terms
4-Quinolones Amino Acid Sequence Anti-Infective Agents/therapeutic use Base Sequence Cloning, Molecular DNA Gyrase DNA Topoisomerases, Type II/genetics Drug Resistance, Microbial/genetics Humans Molecular Sequence Data Point Mutation Serratia Infections/drug therapy,enzymology,genetics Serratia marcescens/drug effects,enzymology,genetics Topoisomerase II Inhibitors
Chemicals
4-Quinolones Anti-Infective Agents Topoisomerase II Inhibitors DNA Gyrase DNA Topoisomerases, Type II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kim J H
Department of Pharmacology and Molecular Biology, C&C Research Laboratories, Kyunggi-do, Korea.
Cho E H
Kim K S
Kim H Y
Kim Y M
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.42.1.190
Published
1998-01-00
Pages
190-3
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC105481
Subset
IM
Databases
GENBANK
U56906
External Links
PubMed source DOI: 10.1128/AAC.42.1.190 PMC Full Text View journal details
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