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PMID: 8384814 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and nucleotide sequence of the Campylobacter jejuni gyrA gene and characterization of quinolone resistance mutations.

Antimicrobial agents and chemotherapy ·Vol. 37 ·No. 3 ·1993-03-00 ·Pages 457-63

Wang Y, Huang WM, Taylor DE

Abstract

The gyrA gene of Campylobacter jejuni UA580, which encodes the A subunit of DNA gyrase, was cloned and its nucleotide sequence was determined. An open reading frame of 2,589 nucleotides was identified, which could code for a polypeptide of 863 amino acids with a M(r) of 97 kDa. Both the nucleotide sequence and the putative amino acid sequence show ca. 50% identity with those of other gyrA genes from gram-positive and gram-negative bacteria. The locations of the gyrA gene on genome maps of both C. jejuni UA580 and Campylobacter coli UA417 were determined. Six nalidixic acid-resistant isolates of C. jejuni were shown to carry mutations in gyrA. Three clinical isolates had Thr-86-to-Ile substitutions. Three laboratory mutants had substitutions of Thr-86 to Ile, Asp-90 to Ala, and Ala-70 to Thr, respectively. The mutation at Thr-86, which is homologous to Ser-83 in Escherichia coli, was associated with high-level resistance to ciprofloxacin in C. jejuni.

Related Genes
gyrA
MeSH Terms
4-Quinolones Amino Acid Sequence Anti-Infective Agents/pharmacology Base Sequence Campylobacter jejuni/chemistry,drug effects,genetics Chromosome Mapping Chromosomes, Bacterial/chemistry Cloning, Molecular DNA Topoisomerases, Type II/genetics,isolation & purification DNA, Bacterial/isolation & purification Drug Resistance, Microbial/genetics Genes, Bacterial Molecular Sequence Data Mutation Topoisomerase II Inhibitors
Chemicals
4-Quinolones Anti-Infective Agents DNA, Bacterial Topoisomerase II Inhibitors DNA Topoisomerases, Type II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang Y
Department of Medical Microbiology, University of Alberta, Edmonton, Canada.
Huang W M
Taylor D E
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35 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.37.3.457
Published
1993-03-00
Pages
457-63
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC187693
Subset
IM
Grants
NIGMS NIH HHS · GM20960 · United States
Databases
GENBANK
L04566, L05008, L05009, L05010, L05011, L05012, L05013, L05014, L08748, M97169
External Links
PubMed source DOI: 10.1128/AAC.37.3.457 PMC Full Text View journal details
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