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PMID: 1649573 Published · ppublish English Journal Article

Spontaneous quinolone resistance in Serratia marcescens due to a mutation in gyrA.

Antimicrobial agents and chemotherapy ·Vol. 35 ·No. 5 ·1991-05-00 ·Pages 898-902

Masecar BL, Robillard NJ

Abstract

Spontaneous quinolone-resistant mutants of MP050, a quinolone-susceptible clinical strain of Serratia marcescens, were isolated on nutrient agar containing 0.5 microgram of ciprofloxacin per ml. One mutant, designated MP051, was selected for further study. Quinolone MICs for MP051 were 4- to 16-fold higher than those for MP050; nonquinolone MICs were unchanged. The DNA gyrase isolated from MP051 was 24-fold less sensitive to inhibition of supercoiling by ciprofloxacin than the DNA gyrase isolated from MP050 was. Inhibition studies on reconstituted combinations of heterologous gyrase subunits showed that the decreased inhibition was dependent on the A subunit of DNA gyrase from MP051. Further evidence that this decreased inhibition was due to a gyrA mutation was provided by analysis of Escherichia coli gyrA gene expression in S. marcescens heterodiploids containing pNJR3-2, a broad-host-range gyrA gene probe. Quinolone susceptibilities of MP051 heterodiploids containing the wild-type E. coli gyrA gene decreased to those of MP050, while quinolone susceptibilities of MP050 containing the same plasmid were unchanged. These results indicate that spontaneous quinolone resistance in MP051 was due to a mutation in gyrA.

Related Genes
gyra
MeSH Terms
Anti-Bacterial Agents/pharmacology Anti-Infective Agents/pharmacology Ciprofloxacin/pharmacology Conjugation, Genetic Culture Media DNA Topoisomerases, Type II/genetics,isolation & purification DNA, Bacterial/analysis,isolation & purification DNA, Superhelical/analysis Drug Resistance, Microbial/genetics Microbial Sensitivity Tests Nalidixic Acid/pharmacology Plasmids Serratia marcescens/drug effects,enzymology,genetics
Chemicals
Anti-Bacterial Agents Anti-Infective Agents Culture Media DNA, Bacterial DNA, Superhelical Nalidixic Acid Ciprofloxacin DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Masecar B L
Pharmaceutical Division, Miles Inc., West Haven, Connecticut 06516.
Robillard N J
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.35.5.898
Published
1991-05-00
Pages
898-902
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC245126
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.35.5.898 PMC Full Text View journal details
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