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PMID: 8849216 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Mutations in gyrA gene of quinolone-resistant Salmonella serotypes isolated from humans and animals.

Antimicrobial agents and chemotherapy ·Vol. 40 ·No. 4 ·1996-04-00 ·Pages 1009-13

Griggs DJ, Gensberg K, Piddock LJ

Abstract

The quinolone resistance-determining regions (QRDRs) of the gyrA genes of quinolone-resistant clinical and veterinary salmonella isolates were sequenced. Substitutions analogous to a substitution of a Ser to a Phe at position 83 (Ser83-->Phe) and Asp87-->Gly or Tyr in Escherichia coli were found, as was a single novel mutation outside of the QRDR resulting in Ala119-->Glu. The data suggest that gyrA mutations are associated with quinolone resistance in veterinary and clinical salmonella isolates and that the limits of the QRDR may require revision.

MeSH Terms
Amino Acid Sequence Animals Anti-Infective Agents Base Sequence DNA Gyrase DNA Topoisomerases, Type II/genetics Drug Resistance, Microbial/genetics Escherichia coli/genetics Fluoroquinolones Humans Molecular Sequence Data Mutation Salmonella/genetics Sequence Homology
Chemicals
Anti-Infective Agents Fluoroquinolones DNA Gyrase DNA Topoisomerases, Type II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Griggs D J
Department of Infection, University of Birmingham, United Kingdom.
Gensberg K
Piddock L J
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28 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.40.4.1009
Published
1996-04-00
Pages
1009-13
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC163248
Subset
IM
Databases
GENBANK
X78977
External Links
PubMed source DOI: 10.1128/AAC.40.4.1009 PMC Full Text View journal details
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