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PMID: 9245791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex.

The Journal of cell biology ·Vol. 138 ·No. 3 ·1997-08-11 ·Pages 629-41

Carminati JL, Stearns T

Abstract

Proper orientation of the mitotic spindle is critical for successful cell division in budding yeast. To investigate the mechanism of spindle orientation, we used a green fluorescent protein (GFP)-tubulin fusion protein to observe microtubules in living yeast cells. GFP-tubulin is incorporated into microtubules, allowing visualization of both cytoplasmic and spindle microtubules, and does not interfere with normal microtubule function. Microtubules in yeast cells exhibit dynamic instability, although they grow and shrink more slowly than microtubules in animal cells. The dynamic properties of yeast microtubules are modulated during the cell cycle. The behavior of cytoplasmic microtubules revealed distinct interactions with the cell cortex that result in associated spindle movement and orientation. Dynein-mutant cells had defects in these cortical interactions, resulting in misoriented spindles. In addition, microtubule dynamics were altered in the absence of dynein. These results indicate that microtubules and dynein interact to produce dynamic cortical interactions, and that these interactions result in the force driving spindle orientation.

MeSH Terms
Cell Division Cytoplasm/physiology,ultrastructure Dyneins/genetics,metabolism Green Fluorescent Proteins Luminescent Proteins Microscopy, Fluorescence Microtubules/chemistry,physiology,ultrastructure Mutation Recombinant Fusion Proteins/analysis Saccharomyces cerevisiae/growth & development,ultrastructure Spindle Apparatus/chemistry,physiology,ultrastructure Tubulin/analysis
Chemicals
Luminescent Proteins Recombinant Fusion Proteins Tubulin Green Fluorescent Proteins Dyneins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Carminati J L
Department of Biological Sciences, Stanford University, Stanford, California 94305-5020, USA.
Stearns T
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.138.3.629
Published
1997-08-11
Pages
629-41
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2141630
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.138.3.629 PMC Full Text View journal details
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