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PMID: 7929558 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle.

The Journal of cell biology ·Vol. 127 ·No. 1 ·1994-10-00 ·Pages 129-38

Clark SW, Meyer DI

Abstract

As part of our ongoing efforts to understand the functional role of vertebrate centractins, we have identified a new member of the actin-related family of proteins in the yeast Saccharomyces cerevisiae using a PCR-based approach. Consistent with the current nomenclature for actin-related proteins in yeast, we propose to denote this locus ACT3. The primary amino acid sequence of Act3p is most similar to canine and human alpha-centractin (73% similarity/54% identity). The sequence of a genomic clone indicates ACT3 lies adjacent to and is transcribed convergently with respect to FUR1 on chromosome VIII. Molecular genetic analysis indicates ACT3 is represented by a single gene from which the corresponding mRNA is expressed at a low level compared to ACT1. Tetrad analysis of heterozygotes harboring a TRP1 replacement of the ACT3-coding region indicates ACT3 is nonessential for growth under normal conditions and at extremes of temperature and osmolarity. However, growth at 14 degrees C indicates a spindle orientation defect similar to phenotypes recently described for yeast harboring mutations in actin, tubulin, or cytoplasmic dynein. Taken together, our data suggest that ACT3 is the S. cerevisiae homologue of vertebrate centractins.

Related Genes
ACT3
MeSH Terms
Actins/genetics,physiology Amino Acid Sequence Base Sequence Cell Division Cloning, Molecular DNA, Fungal/analysis Fungal Proteins/genetics,physiology Genes, Fungal/genetics Molecular Sequence Data Nuclear Proteins Phylogeny RNA, Fungal/analysis RNA, Messenger/analysis Saccharomyces cerevisiae/cytology,genetics,growth & development Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Analysis, DNA Sequence Deletion/physiology Sequence Homology, Amino Acid Spindle Apparatus Spores, Fungal
Chemicals
Actins Arp4 protein, S cerevisiae DNA, Fungal Fungal Proteins Nuclear Proteins RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Proteins centractin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clark S W
Department of Biological Chemistry, University of California, Los Angeles 90024.
Meyer D I
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.127.1.129
Published
1994-10-00
Pages
129-38
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120173
Subset
IM
Grants
NIGMS NIH HHS · GM07185 · United States
Databases
GENBANK
X79811
External Links
PubMed source DOI: 10.1083/jcb.127.1.129 PMC Full Text View journal details
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