A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors.

Nucleic acids research ·Vol. 19 ·No. 5 ·1991-03-11 ·Pages 1156

Holton TA, Graham MW

Abstract

暂无摘要

MeSH Terms

Cloning, Molecular/methods Dideoxynucleotides Genetic Vectors Polymerase Chain Reaction Thymine Nucleotides

Chemicals

Dideoxynucleotides Thymine Nucleotides 2',3'-dideoxythymidine triphosphate

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Holton T A

Calgene Pacific Pty. Ltd., Collingwood, Victoria, Australia.

Graham M W

References (2)

2 references, click to expand

Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.
Nucleic Acids Res. 1988 Oct 25;16(20):9677-86 PMID: 2460825
Restriction endonuclease cleavage at the termini of PCR products.
Biotechniques. 1990 Sep;9(3):304, 306 PMID: 2171587

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

Published

1991-03-11

Pages

1156

Language

English

Region

England

NLM ID

0411011

PMCID

PMC333802

Subset

External Links

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