Abstract
The NUM1 gene is involved in the control of nuclear migration in Saccharomyces cerevisiae. The content of NUM1 mRNA fluctuates during the cell cycle, reaching a maximum at S/G2 phase, and the translation product Num1p associates with the cortex of mother cells mainly during S, G2, and mitosis, as seen by indirect immunofluorescence. The nuclear spindle in NUM1-deficient large-budded cells often fails to align along the mother/bud axis, while abnormally elongated astral microtubules emanate from both spindle pole bodies. A num1 null mutation confers temperature sensitivity to the cold-sensitive alpha-tubulin mutant tub1-1, and shows synthetic lethality with the beta-tubulin mutant alleles tub2-402, tub2-403, tub2-404, and tub2-405. Deletion mapping has defined three functionally important Num1p regions: a potential EF hand Ca2+ binding site, a cluster of potential phosphorylation sites and a pleckstrin homology domain. The latter domain appears to be involved in targeting Num1p to the mother cell cortex. Our data suggest that the periodically expressed NUM1 gene product controls nuclear migration by affecting astral microtubule functions.
MeSH Terms
Calcium-Binding Proteins/genetics,metabolism,ultrastructure
Cell Membrane/metabolism
Cell Nucleus/physiology
Cytoskeletal Proteins
Fluorescent Antibody Technique, Indirect
Fungal Proteins/genetics,metabolism,ultrastructure
G2 Phase/physiology
Microscopy, Phase-Contrast
Microtubules/physiology,ultrastructure
Mutation/physiology
Periodicity
RNA, Messenger/metabolism
S Phase/physiology
Saccharomyces cerevisiae/genetics,metabolism
Saccharomyces cerevisiae Proteins
Spindle Apparatus/physiology
Tubulin/genetics
Chemicals
Calcium-Binding Proteins
Cytoskeletal Proteins
Fungal Proteins
NUM1 protein, S cerevisiae
RNA, Messenger
Saccharomyces cerevisiae Proteins
Tubulin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Farkasovsky M
Max-Planck-Institut für experimentelle Medizin, Göttingen, Federal Republic of Germany.
Küntzel H
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