Domains of the Saccharomyces cerevisiae CDC25 gene controlling mitosis and meiosis.

Molecular & general genetics : MGG ·Vol. 214 ·No. 2 ·1988-10-00 ·Pages 271-7

Munder T, Mink M, Küntzel H

Abstract

The cell division cycle gene CDC25 was replaced by various disrupted and deleted mutant copies. Mutants disrupted at a central position of the gene, or lacking 532 residues within the amino-terminal half of the gene product grow normally in glucose, but not in acetate media, and they fail to sporulate as homozygous diploids. Disruptions or deletions within the carboxy-terminal half are lethal, except for the deletion of the 38 carboxy-terminal residues, which are required for sporulation but not for growth in glucose or acetate media. It is concluded that distinct domains of the CDC25 gene product are involved in the control of mitosis and/or meiosis.

MeSH Terms

Chromosome Deletion Crosses, Genetic Genes, Fungal Genotype Meiosis Mitosis Mutation Plasmids Saccharomyces cerevisiae/cytology,genetics Spores, Fungal/cytology

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Munder T

Max-Planck-Institut für Experimentelle Medizin, Abteilung Chemie, Göttingen, Federal Republic of Germany.

Mink M

Küntzel H

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00337721

Published

1988-10-00

Pages

271-7

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00337721

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Domains of the Saccharomyces cerevisiae CDC25 gene controlling mitosis and meiosis.

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