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PMID: 8134356 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cytoplasmic dynein is involved in nuclear migration in Aspergillus nidulans.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 6 ·1994-03-15 ·Pages 2100-4

Xiang X, Beckwith SM, Morris NR

Abstract

Nuclear migration plays an important role in the growth and development of many organisms including the multinuclear fungus Aspergillus nidulans. We have identified four genes, nudA, nudC, nudF, and nudG, in which temperature-sensitive mutations affect nuclear distribution. In this report, we describe the cloning of the nudA gene by complementation of the mutant phenotype by using a chromosome VIII-specific cosmid library. A genomic fragment of nudA hybridized to an mRNA of approximately 14 kb. Sequencing analysis of nudA revealed four ATP-binding sites that are characteristic of the cytoplasmic dynein heavy chain. The amino acid sequence of the nudA gene product shows 52% overall identity with the rat brain cytoplasmic dynein heavy chain. Our study provides in vivo evidence that dynein, a microtubule motor molecule, plays a role in the nuclear migration process.

Related Genes
nudA nudC nudF nudG
MeSH Terms
Adenosine Triphosphate/metabolism Amino Acid Sequence Animals Aspergillus nidulans/genetics,growth & development Cell Nucleus/metabolism Cloning, Molecular Cytoplasm/metabolism Dyneins/genetics,metabolism Genes, Fungal Genetic Complementation Test Molecular Sequence Data Mutation Phenotype Restriction Mapping Sequence Homology, Amino Acid Temperature
Chemicals
Adenosine Triphosphate Dyneins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Xiang X
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Department of Pharmacology, Piscataway 08854-5635.
Beckwith S M
Morris N R
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.6.2100
Published
1994-03-15
Pages
2100-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43317
Subset
IM
Databases
GENBANK
U03904
External Links
PubMed source DOI: 10.1073/pnas.91.6.2100 PMC Full Text View journal details
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