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PMID: 2143999 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Brain dynein (MAP1C) localizes on both anterogradely and retrogradely transported membranous organelles in vivo.

The Journal of cell biology ·Vol. 111 ·No. 3 ·1990-09-00 ·Pages 1027-37

Hirokawa N, Sato-Yoshitake R, Yoshida T, Kawashima T

Abstract

Brain dynein is a microtubule-activated ATPase considered to be a candidate to function as a molecular motor to transport membranous organelles retrogradely in the axon. To determine whether brain dynein really binds to retrogradely transported organelles in vivo and how it is transported to the nerve terminals, we studied the localization of brain dynein in axons after the ligation of peripheral nerves by light and electron microscopic immunocytochemistry using affinity-purified anti-brain dynein antibodies. Different classes of organelles preferentially accumulated at the regions proximal and distal to the ligated part. Interestingly, brain dynein accumulated both at the regions proximal and distal to the ligation sites and localized not only on retrogradely transported membranous organelles but also on anterogradely transported ones. This is the first evidence to show that brain dynein associates with retrogradely transported organelles in vivo and that brain dynein is transported to the nerve terminal by fast flow. This also suggests that there may be some mechanism that activates brain dynein only for retrograde transport.

MeSH Terms
Adenosine Triphosphatases/metabolism Animals Axons/metabolism,ultrastructure Biological Transport/physiology Brain/ultrastructure Cattle Dyneins/metabolism Female Ligation Mice Neurons/metabolism,ultrastructure Organelles/metabolism,ultrastructure Peripheral Nerves/physiology
Chemicals
Adenosine Triphosphatases Dyneins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hirokawa N
Department of Anatomy and Cell Biology, School of Medicine, University of Tokyo, Japan.
Sato-Yoshitake R
Yoshida T
Kawashima T
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.3.1027
Published
1990-09-00
Pages
1027-37
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116262
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.111.3.1027 PMC Full Text View journal details
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