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PMID: 9184221 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme.

The EMBO journal ·Vol. 16 ·No. 10 ·1997-05-15 ·Pages 2756-68

Duong F, Wickner W

Abstract

Escherichia coli preprotein translocase contains a membrane-embedded trimeric complex of SecY, SecE and SecG (SecYEG) and the peripheral SecA protein. SecYE is the conserved functional 'core' of the SecYEG complex. Although sufficient to provide sites for high-affinity binding and membrane insertion of SecA, and for its activation as a preprotein-dependent ATPase, SecYE has only very low capacity to support translocation. The proteins encoded by the secD operon--SecD, SecF and YajC--also form an integral membrane heterotrimeric complex (SecDFyajC). Physical and functional studies show that these two trimeric complexes are associated to form SecYEGDFyajC, the hexameric integral membrane domain of the preprotein translocase 'holoenzyme'. Either SecG or SecDFyajC can support the translocation activity of SecYE by facilitating the ATP-driven cycle of SecA membrane insertion and de-insertion at different stages of the translocation reaction. Our findings show that each of the prokaryote-specific subunits (SecA, SecG and SecDFyajC) function together to promote preprotein movement at the SecYE core of the translocase.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Adenosine Triphosphate/metabolism Bacterial Proteins/genetics,metabolism Biological Transport Coenzymes/genetics,metabolism Escherichia coli/enzymology,genetics,metabolism Escherichia coli Proteins Hydrolysis Macromolecular Substances Membrane Proteins Membrane Transport Proteins Precipitin Tests Protein Binding Protein Precursors/metabolism SEC Translocation Channels SecA Proteins
Chemicals
Bacterial Proteins Coenzymes Escherichia coli Proteins Macromolecular Substances Membrane Proteins Membrane Transport Proteins Protein Precursors SEC Translocation Channels SecD protein, E coli SecE protein, E coli SecF protein, E coli SecG protein, E coli SecY protein, E coli secD protein, Bacteria secF protein, Bacteria Adenosine Triphosphate Adenosine Triphosphatases SecA Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Duong F
Dartmouth Medical School, Department of Biochemistry, Hanover, NH 03755, USA.
Wickner W
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45 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.10.2756
Published
1997-05-15
Pages
2756-68
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1169885
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.10.2756 PMC Full Text View journal details
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