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PMID: 8021192 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SecF stabilizes SecD and SecY, components of the protein translocation machinery of the Escherichia coli cytoplasmic membrane.

Journal of bacteriology ·Vol. 176 ·No. 13 ·1994-07-00 ·Pages 4111-6

Sagara K, Matsuyama S, Mizushima S

Abstract

The effect of the overproduction of SecF encoded by the tac-secF gene on a plasmid on the synthesis of other Sec proteins was studied in Escherichia coli. SecF overproduction resulted in the simultaneous overproduction of SecD encoded by the tac-secD gene on a plasmid. Deletion of the orf6 gene, located downstream of the secF gene, had no effect on SecD overproduction. A pulse-chase experiment revealed that the overproduction was due to stabilization of SecD with SecF. SecF overproduction also resulted in the overproduction of SecY encoded by the tac-secY gene on a plasmid as well. SecF overproduction also enhanced the level of SecY expressed by the chromosomal secY gene. This SecF effect was not due to its effect on SecD or SecE, since SecF overproduction did not affect the levels of SecD and SecE expressed by the chromosomal secD and secE genes, respectively. SecE-dependent overproduction of SecY has already been demonstrated. It is suggested that SecF interacts with both SecD and SecY. SecE-SecY interaction has been demonstrated. It is likely, therefore, that all Sec proteins in the cytoplasmic membrane interact with each other.

MeSH Terms
Bacterial Proteins/genetics,metabolism Biological Transport Cell Membrane/metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Membrane Proteins Membrane Transport Proteins Models, Biological Open Reading Frames/genetics Plasmids/genetics Recombinant Proteins/metabolism SEC Translocation Channels Sequence Deletion
Chemicals
Bacterial Proteins Escherichia coli Proteins Membrane Proteins Membrane Transport Proteins Recombinant Proteins SEC Translocation Channels SecD protein, E coli SecF protein, E coli SecY protein, E coli secD protein, Bacteria secF protein, Bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sagara K
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Matsuyama S
Mizushima S
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35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.13.4111-4116.1994
Published
1994-07-00
Pages
4111-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205610
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.13.4111-4116.1994 PMC Full Text View journal details
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