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PMID: 2170107 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The secD locus of E.coli codes for two membrane proteins required for protein export.

The EMBO journal ·Vol. 9 ·No. 10 ·1990-10-00 ·Pages 3209-16

Gardel C, Johnson K, Jacq A, Beckwith J

Abstract

Cold-sensitive mutations in the secD locus of Escherichia coli result in severe defects in protein export at the non-permissive temperature of 23 degrees C. DNA sequence of a cloned fragment that includes the secD locus reveals open reading frames for seven polypeptide chains. Both deletions and TnphoA insertions in this clone have been used in maxicell and complementation studies to define the secD locus and its products. The secD mutations fall into two complementation groups, defining genes we have named secD and secF. These two genes comprise an operon, the first case of two genes involved in the export process being co-transcribed. The DNA sequence of the two genes along with alkaline phosphatase fusion analysis indicates that they code for integral proteins of the cytoplasmic membrane. We suggest that these two proteins may form a complex in the membrane which acts at late steps in the export process.

Related Genes
secD secF
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Base Sequence Chromosome Deletion Chromosomes, Bacterial Cloning, Molecular Cold Temperature DNA Transposable Elements Escherichia coli/genetics,metabolism Genetic Complementation Test Membrane Proteins/genetics,metabolism Molecular Sequence Data Mutation Operon Plasmids Protein Conformation Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins DNA Transposable Elements Membrane Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gardel C
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Johnson K
Jacq A
Beckwith J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-10-00
Pages
3209-16
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552051
Subset
IM
Databases
GENBANK
X56175
Corrections
ErratumIn
PMID: 2249673
External Links
PubMed source PMC Full Text View journal details
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