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PMID: 2202721 Published · ppublish English Journal Article Review

The sec and prl genes of Escherichia coli.

Journal of bioenergetics and biomembranes ·Vol. 22 ·No. 3 ·1990-06-00 ·Pages 291-310

Bieker KL, Phillips GJ, Silhavy TJ

Abstract

Two general approaches have been used to define genetically the genes that encode components of the cellular protein export machinery. One of these strategies identifies mutations that confer a conditional-lethal, pleiotropic export defect (sec, secretion). The other identifies dominant suppressors of signal sequence mutations (prl, protein localization). Subsequent characterization reveals that in at least three cases, prlA/secY, prlD/secA, and prlG/secE, both types of mutations are found within the same structural gene. This convergence is satisfying and provides compelling evidence for direct involvement of these gene products in the export process.

MeSH Terms
Bacterial Proteins/genetics,metabolism Escherichia coli/genetics,metabolism Mutation Protein Sorting Signals/genetics Suppression, Genetic
Chemicals
Bacterial Proteins Protein Sorting Signals
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bieker K L
Department of Biology, Princeton University, New Jersey 08544.
Phillips G J
Silhavy T J
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Article Info
Journal
Journal of bioenergetics and biomembranes
Abbr.
J Bioenerg Biomembr
ISSN
0145-479X
DOI
10.1007/BF00763169
Published
1990-06-00
Pages
291-310
Language
English
Region
United States
NLM ID
7701859
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00763169 View journal details
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