Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 4 ·1977-04-00 ·Pages 1440-4

Inouye H, Beckwith J

Abstract

Alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] of E. coli was synthesized in a cell-free system, and the size of the direct translation product was analyzed. The product has a higher molecular weight than the mature alkaline phosphatase found in the periplasm. The direct translation product can be processed to the mature size by an E. coli membrane fraction; the processing activity copurifies with the outer-membrane fraction. The presumed precursor can dimerize to form active enzyme without being processed, and the resultant enzyme appears to be more hydrophobic than the mature enzyme. These findings are discussed in connection with the "signal hypothesis" proposed for the excretion of proteins across membranes.

MeSH Terms

Alkaline Phosphatase/biosynthesis Cell Membrane/enzymology Enzyme Precursors/biosynthesis Escherichia coli/enzymology Galactosidases/biosynthesis Genes Protein Biosynthesis

Chemicals

Enzyme Precursors Alkaline Phosphatase Galactosidases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Inouye H

Beckwith J

References (24)

24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.4.1440

Published

1977-04-00

Pages

1440-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430790

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.4.1440 PMC Full Text View journal details

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Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro.

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