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PMID: 7507921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic and molecular characterization of the Escherichia coli secD operon and its products.

Journal of bacteriology ·Vol. 176 ·No. 3 ·1994-02-00 ·Pages 804-14

Pogliano KJ, Beckwith J

Abstract

The secD operon of Escherichia coli is required for the efficient export of proteins. We have characterized this operon, and found that, in addition to secD and secF, it contains the upstream gene yajC, but not the genes queA or tgt, in contrast to previous reports. An analysis of yajC mutations constructed in vitro and recombined onto the chromosome indicates that yajC is neither essential nor a sec gene. The secD operon is not induced in response to either secretion defects or temperature changes. TnphoA fusions have been used to analyze the topology of SecD in the inner membrane; the protein contains six transmembrane stretches and a large periplasmic domain. TnphoA fusions to SecD and SecF have also been recombined onto the chromosome and used to determine the level of these proteins within the cell. Our results indicate that there are fewer than 30 SecD and SecF molecules per cell.

Related Genes
queA secD secF tgt yajC yajD
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Escherichia coli/genetics Escherichia coli Proteins Gene Expression Genes, Bacterial Hydrogen Bonding Membrane Proteins/genetics Membrane Transport Proteins Molecular Sequence Data Mutagenesis, Insertional Nucleic Acid Conformation Oligonucleotide Probes/chemistry Operon RNA, Bacterial/genetics RNA, Messenger/genetics Temperature
Chemicals
Bacterial Proteins Escherichia coli Proteins Membrane Proteins Membrane Transport Proteins Oligonucleotide Probes RNA, Bacterial RNA, Messenger SecD protein, E coli SecF protein, E coli secD protein, Bacteria secF protein, Bacteria
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pogliano K J
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Beckwith J
References (43)
43 references, click to expand
  1. Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.
    J Bacteriol. 1983 Apr;154(1):366-74 PMID: 6339478
  2. Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):653-7 PMID: 6187001
  3. The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review.
    Gene. 1984 Feb;27(2):131-49 PMID: 6327463
  4. Evidence for specificity at an early step in protein export in Escherichia coli.
    J Bacteriol. 1985 Jul;163(1):267-74 PMID: 3891730
  5. Suppression of growth and protein secretion defects in Escherichia coli secA mutants by decreasing protein synthesis.
    J Bacteriol. 1986 Jun;166(3):878-83 PMID: 3519584
  6. A genetic approach to analyzing membrane protein topology.
    Science. 1986 Sep 26;233(4771):1403-8 PMID: 3529391
  7. Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives.
    Mol Gen Genet. 1986 Nov;205(2):285-90 PMID: 3100913
  8. secD, a new gene involved in protein export in Escherichia coli.
    J Bacteriol. 1987 Mar;169(3):1286-90 PMID: 3029032
  9. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  10. Substitution of tyrosine for either cysteine in beta-lactamase prevents release from the membrane during secretion.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8540-3 PMID: 3317414
  11. A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene.
    Genetics. 1988 Apr;118(4):571-9 PMID: 3284784
  12. Regulation of the Escherichia coli secA gene by protein secretion defects: analysis of secA, secB, secD, and secY mutants.
    J Bacteriol. 1988 Jul;170(7):3281-2 PMID: 2838466
  13. Effects of Escherichia coli secB mutations on pre-maltose binding protein conformation and export kinetics.
    J Biol Chem. 1988 Aug 15;263(23):11554-8 PMID: 3042772
  14. SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8953-7 PMID: 2848248
  15. Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide.
    J Bacteriol. 1989 Jan;171(1):402-9 PMID: 2536662
  16. Chromosomal transformation of Escherichia coli recD strains with linearized plasmids.
    J Bacteriol. 1989 May;171(5):2609-13 PMID: 2651408
  17. Translocation of pro-OmpA across inner membrane vesicles of Escherichia coli occurs in two consecutive energetically distinct steps.
    J Biol Chem. 1989 Oct 5;264(28):16465-9 PMID: 2674133
  18. Electrochemical potential releases a membrane-bound secretion intermediate of maltose-binding protein in Escherichia coli.
    J Bacteriol. 1990 Sep;172(9):4870-6 PMID: 2203734
  19. Minimal deletion of amino acids from the carboxyl terminus of the B subunit of heat-labile enterotoxin causes defects in its assembly and release from the cytoplasmic membrane of Escherichia coli.
    J Biol Chem. 1990 Sep 5;265(25):15239-44 PMID: 2203772
  20. The secD locus of E.coli codes for two membrane proteins required for protein export.
    EMBO J. 1990 Oct;9(10):3209-16 PMID: 2170107
  21. Escherichia coli sec mutants accumulate a processed immature form of maltose-binding protein (MBP), a late-phase intermediate in MBP export.
    J Bacteriol. 1990 Oct;172(10):5643-9 PMID: 2211501
  22. Genetic analysis of membrane protein topology by a sandwich gene fusion approach.
    Proc Natl Acad Sci U S A. 1990 Oct;87(19):7574-8 PMID: 2170984
  23. Characterization of cold-sensitive secY mutants of Escherichia coli.
    J Bacteriol. 1990 Dec;172(12):7005-10 PMID: 2254269
  24. Transposition of the Lac region of E. coli.
    Cold Spring Harb Symp Quant Biol. 1966;31:393-401 PMID: 4866389
  25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  26. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
    J Mol Biol. 1976 Jul 5;104(3):541-55 PMID: 781293
  27. Cloned structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12: localization on hybrid plasmid pTU100 and expression of a fragment of the gene.
    Mol Gen Genet. 1980;179(1):13-20 PMID: 6256604
  28. Suppressor mutations that restore export of a protein with a defective signal sequence.
    Cell. 1981 Jan;23(1):79-88 PMID: 7011570
  29. The FtsQ protein of Escherichia coli: membrane topology, abundance, and cell division phenotypes due to overproduction and insertion mutations.
    J Bacteriol. 1991 Apr;173(7):2187-95 PMID: 2007547
  30. Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes.
    J Bacteriol. 1991 Apr;173(7):2256-64 PMID: 1706703
  31. One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery.
    EMBO J. 1991 Jul;10(7):1749-57 PMID: 2050112
  32. Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.
    Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6545-9 PMID: 1830665
  33. The enzymology of protein translocation across the Escherichia coli plasma membrane.
    Annu Rev Biochem. 1991;60:101-24 PMID: 1831965
  34. SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase.
    J Biol Chem. 1992 Feb 25;267(6):4166-70 PMID: 1531482
  35. Overproduction, purification and characterization of SecD and SecF, integral membrane components of the protein translocation machinery of Escherichia coli.
    Biochim Biophys Acta. 1992 Jul 13;1122(1):77-84 PMID: 1633199
  36. The promoter of the tgt/sec operon in Escherichia coli is preceded by an upstream activation sequence that contains a high affinity FIS binding site.
    Nucleic Acids Res. 1992 Aug 25;20(16):4193-8 PMID: 1508713
  37. FtsL, an essential cytoplasmic membrane protein involved in cell division in Escherichia coli.
    J Bacteriol. 1992 Dec;174(23):7716-28 PMID: 1332942
  38. High copy number of the pUC plasmid results from a Rom/Rop-suppressible point mutation in RNA II.
    Mol Microbiol. 1992 Nov;6(22):3385-93 PMID: 1283002
  39. SecD is involved in the release of translocated secretory proteins from the cytoplasmic membrane of Escherichia coli.
    EMBO J. 1993 Jan;12(1):265-70 PMID: 8428584
  40. The Cs sec mutants of Escherichia coli reflect the cold sensitivity of protein export itself.
    Genetics. 1993 Apr;133(4):763-73 PMID: 8462840
  41. E. coli mutant pleiotropically defective in the export of secreted proteins.
    Cell. 1981 Sep;25(3):765-72 PMID: 7026050
  42. Regulation of a membrane component required for protein secretion in Escherichia coli.
    Cell. 1982 Aug;30(1):311-9 PMID: 6751561
  43. Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
    Methods Enzymol. 1983;100:255-66 PMID: 6194404
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.3.804-814.1994
Published
1994-02-00
Pages
804-14
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205118
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.3.804-814.1994 PMC Full Text View journal details
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