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PMID: 3100913 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives.

Molecular & general genetics : MGG ·Vol. 205 ·No. 2 ·1986-11-00 ·Pages 285-90

Lopilato J, Bortner S, Beckwith J

Abstract

We describe mutants of Escherichia coli that decrease the plasmid copy number of pBR322 derivatives. One mutant was partially characterized genetically and its mutation, designated pcnB for plasmid copy number, was mapped to approximately 3 min on the E. coli chromosome. This locus is distinct from other genes whose products are known to affect plasmid replication or stable plasmid maintenance. The pcnB mutant strain should be useful for cloning genes into pBR322 that have aberrant or deleterious effects on the cell when present in high copy number.

MeSH Terms
Chromosomes, Bacterial/physiology Escherichia coli/drug effects,enzymology,genetics Genes, Bacterial Mutation Nitrosoguanidines/pharmacology Plasmids beta-Galactosidase/genetics,metabolism beta-Lactamases/genetics,metabolism
Chemicals
Nitrosoguanidines beta-Galactosidase beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lopilato J
Bortner S
Beckwith J
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27 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00430440
Published
1986-11-00
Pages
285-90
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM13017 · United States
External Links
PubMed source DOI: 10.1007/BF00430440
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