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PMID: 9106367 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Review

RNA editing in kinetoplastid protozoa.

Microbiology and molecular biology reviews : MMBR ·Vol. 61 ·No. 1 ·1997-03-00 ·Pages 105-20

Stuart K, Allen TE, Heidmann S, Seiwert SD

Abstract

Mitochondrial transcripts in kinetoplastids undergo remarkable posttranscriptional editing by uridylate insertion and deletion. The often dramatic remodeling of pre-mRNA sequences is directed by small guide RNAs (gRNAs) to produce mature mRNAs. In vitro analyses of editing have been used to determine the mechanism of editing and show that editing occurs by a series of enzyme-catalyzed steps. They also show that chimeric gRNA/mRNA molecules are not editing intermediates as proposed but are aberrant end products of editing. The complexes and molecules that catalyze editing are now being identified and characterized. The origin of editing, its developmental regulation which helps control the switching between terminal respiratory systems during the life cycle of trypanosomes, and other areas for future study are discussed.

MeSH Terms
Animals Base Sequence Biological Evolution Chimera Endoribonucleases/metabolism Eukaryota/genetics,metabolism Kinetoplastida/genetics,metabolism Models, Genetic Models, Structural RNA Editing RNA Precursors/metabolism RNA, Guide/metabolism RNA, Protozoan/metabolism
Chemicals
RNA Precursors RNA, Guide RNA, Protozoan Endoribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stuart K
Seattle Biomedical Research Institute, Washington 98109-1651, USA. kstuart@u.washington.edu
Allen T E
Heidmann S
Seiwert S D
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Article Info
Journal
Microbiology and molecular biology reviews : MMBR
Abbr.
Microbiol Mol Biol Rev
ISSN
1092-2172
DOI
10.1128/mmbr.61.1.105-120.1997
Email
kstuart@u.washington.edu
Published
1997-03-00
Pages
105-20
Language
English
Region
United States
NLM ID
9706653
PMCID
PMC232603
Subset
IM
Grants
NIAID NIH HHS · 1F32AI09206-01 · United States
NIAID NIH HHS · AI14102 · United States
NIGMS NIH HHS · GM42188 · United States
External Links
PubMed source DOI: 10.1128/mmbr.61.1.105-120.1997 PMC Full Text View journal details
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