Home LiteratureArticle Details
PMID: 1370342 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An intergenic G-rich region in Leishmania tarentolae kinetoplast maxicircle DNA is a pan-edited cryptogene encoding ribosomal protein S12.

Molecular and cellular biology ·Vol. 12 ·No. 1 ·1992-01-00 ·Pages 56-67

Maslov DA, Sturm NR, Niner BM, Gruszynski ES, Peris M, Simpson L

Abstract

Six short G-rich intergenic regions in the maxicircle of Leishmania tarentolae are conserved in location and polarity in two other kinetoplastid species. We show here that G-rich region 6 (G6) represents a pan-edited cryptogene which contains at least two domains edited independently in a 3'-to-5' manner connected by short unedited regions. In the completely edited RNA, 117 uridines are added at 49 sites and 32 uridines are deleted at 13 sites, creating a translated 85-amino-acid polypeptide. Similar polypeptides are probably encoded by pan-edited G6 transcripts in two other species. The G6 polypeptide has significant sequence similarity to the family of S12 ribosomal proteins. A minicircle-encoded gRNA overlaps 12 editing sites in G6 mRNA, and chimeric gRNA/mRNA molecules were shown to exist, in agreement with the transesterification model for editing.

MeSH Terms
Amino Acid Sequence Animals Base Composition Base Sequence Blotting, Northern Cells, Cultured DNA, Circular/genetics DNA, Kinetoplast DNA, Protozoan/genetics Guanine Introns Leishmania/genetics Molecular Sequence Data Poly A RNA RNA Processing, Post-Transcriptional RNA, Guide RNA, Protozoan/genetics,metabolism Ribosomal Proteins/genetics Sequence Alignment
Chemicals
DNA, Circular DNA, Kinetoplast DNA, Protozoan RNA, Guide RNA, Protozoan Ribosomal Proteins ribosomal protein S12 Poly A Guanine RNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Maslov D A
Department of Biology, University of California, Los Angeles 90024.
Sturm N R
Niner B M
Gruszynski E S
Peris M
Simpson L
References (49)
49 references, click to expand
  1. Isolation of maxicircle component of kinetoplast DNA from hemoflagellate protozoa.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1585-8 PMID: 286999
  2. A model for RNA editing in kinetoplastid mitochondria: "guide" RNA molecules transcribed from maxicircle DNA provide the edited information.
    Cell. 1990 Jan 26;60(2):189-98 PMID: 1688737
  3. RNA editing involves indiscriminate U changes throughout precisely defined editing domains.
    Cell. 1990 Jun 15;61(6):1001-11 PMID: 1693545
  4. Trypanosoma equiperdum minicircles encode three distinct primary transcripts which exhibit guide RNA characteristics.
    Mol Cell Biol. 1991 Mar;11(3):1668-75 PMID: 1825348
  5. Short leader sequences may be transferred from small RNAs to pre-mature mRNAs by trans-splicing in Euglena.
    EMBO J. 1991 Sep;10(9):2621-5 PMID: 1868836
  6. The cyanelle str operon from Cyanophora paradoxa: sequence analysis and phylogenetic implications.
    Plant Mol Biol. 1990 Oct;15(4):561-73 PMID: 2129337
  7. Partially edited mRNAs for cytochrome b and subunit III of cytochrome oxidase from Leishmania tarentolae mitochondria: RNA editing intermediates.
    Cell. 1990 Jun 1;61(5):871-8 PMID: 2160860
  8. The mitochondrial tRNAs of Trypanosoma brucei are nuclear encoded.
    J Biol Chem. 1990 Nov 5;265(31):19208-15 PMID: 2229071
  9. The MURF3 gene of T. brucei contains multiple domains of extensive editing and is homologous to a subunit of NADH dehydrogenase.
    Cell. 1990 Sep 7;62(5):901-11 PMID: 2393904
  10. Editing of kinetoplastid mitochondrial mRNAs by uridine addition and deletion generates conserved amino acid sequences and AUG initiation codons.
    Cell. 1988 May 6;53(3):401-11 PMID: 2452696
  11. Interaction of ribosomal proteins S5, S6, S11, S12, S18 and S21 with 16 S rRNA.
    J Mol Biol. 1988 Jun 20;201(4):683-95 PMID: 2459389
  12. RNA editing: the creation of nucleotide sequences in mRNA--a minireview.
    Gene. 1989 Oct 15;82(1):155-60 PMID: 2684771
  13. Kinetoplastid mitochondria contain functional tRNAs which are encoded in nuclear DNA and also contain small minicircle and maxicircle transcripts of unknown function.
    Nucleic Acids Res. 1989 Jul 25;17(14):5427-45 PMID: 2762144
  14. Characterization of cytochrome c oxidase III transcripts that are edited only in the 3' region.
    Cell. 1988 Oct 21;55(2):267-72 PMID: 2844415
  15. Phylogenetic meaning of the kingdom concept: an unusual ribosomal RNA from Giardia lamblia.
    Science. 1989 Jan 6;243(4887):75-7 PMID: 2911720
  16. Comparison of the maxicircle (mitochondrial) genomes of Leishmania tarentolae and Trypanosoma brucei at the level of nucleotide sequence.
    J Biol Chem. 1987 May 5;262(13):6182-96 PMID: 3032958
  17. A minimal ribosomal RNA: sequence and secondary structure of the 9S kinetoplast ribosomal RNA from Leishmania tarentolae.
    Proc Natl Acad Sci U S A. 1985 Mar;82(5):1401-5 PMID: 3856267
  18. Synchronization of Leishmania tarentolae by hydroxyurea.
    J Protozool. 1970 Nov;17(4):511-7 PMID: 4101133
  19. Los Alamos sequence analysis package for nucleic acids and proteins.
    Nucleic Acids Res. 1982 Jan 11;10(1):183-96 PMID: 6174934
  20. Sequence heterogeneity and anomalous electrophoretic mobility of kinetoplast minicircle DNA from Leishmania tarentolae.
    Gene. 1984 Mar;27(3):265-77 PMID: 6329906
  21. DNA sequences from the str operon of Escherichia coli.
    J Biol Chem. 1980 May 25;255(10):4660-6 PMID: 6989816
  22. Kinetoplast RNA of Leishmania tarentolae.
    Cell. 1978 May;14(1):169-78 PMID: 667932
  23. RNA editing in transcripts of the mitochondrial genes of the insect trypanosome Crithidia fasciculata.
    EMBO J. 1990 Jan;9(1):257-62 PMID: 1688530
  24. Kinetoplast DNA minicircles encode guide RNAs for editing of cytochrome oxidase subunit III mRNA.
    Cell. 1990 Jun 1;61(5):879-84 PMID: 1693097
  25. RNA editing--a novel genetic phenomenon?
    Science. 1990 Oct 26;250(4980):512-3 PMID: 1700474
  26. Chimeric gRNA-mRNA molecules with oligo(U) tails covalently linked at sites of RNA editing suggest that U addition occurs by transesterification.
    Cell. 1991 May 17;65(4):543-50 PMID: 1709591
  27. The two ATPase 6 mRNAs of Leishmania tarentolae differ at their 3' ends.
    Mol Biochem Parasitol. 1991 Oct;48(2):139-49 PMID: 1837067
  28. Comparative biochemistry and biophysics of ribosomal proteins.
    Int Rev Cytol. 1991;124:103-36 PMID: 2001915
  29. An extensively edited mitochondrial transcript in kinetoplastids encodes a protein homologous to ATPase subunit 6.
    Cell. 1990 Jun 1;61(5):885-94 PMID: 2140530
  30. The nucleotide sequence of the 12S ribosomal RNA gene from kinetoplast DNA of the protozoan Crithidia oncopelti.
    Nucleic Acids Res. 1990 May 11;18(9):2811 PMID: 2160071
  31. Organization of minicircle genes for guide RNAs in Trypanosoma brucei.
    Cell. 1990 Nov 16;63(4):783-90 PMID: 2171782
  32. Nucleotide sequence of the mitochondrial genome of Paramecium.
    Nucleic Acids Res. 1990 Jan 11;18(1):173-80 PMID: 2308823
  33. Profile analysis.
    Methods Enzymol. 1990;183:146-59 PMID: 2314273
  34. Variation of G-rich mitochondrial transcripts among stocks of Trypanosoma brucei.
    Mol Biochem Parasitol. 1987 Jan 15;22(2-3):259-72 PMID: 2437452
  35. Extensive editing of the cytochrome c oxidase III transcript in Trypanosoma brucei.
    Cell. 1988 May 6;53(3):413-22 PMID: 2452697
  36. RNA-editing in trypanosome mitochondria.
    Biochim Biophys Acta. 1989 Mar 1;1007(2):131-9 PMID: 2465776
  37. RNA editing and the mitochondrial cryptogenes of kinetoplastid protozoa.
    Cell. 1989 May 5;57(3):355-66 PMID: 2470509
  38. The evolutionary origins of organelles.
    Trends Genet. 1989 Sep;5(9):294-9 PMID: 2686121
  39. Chloroplast ribosomal protein gene rps12 of Chlamydomonas reinhardtii. Wild-type sequence, mutation to streptomycin resistance and dependence, and function in Escherichia coli.
    J Biol Chem. 1989 Sep 25;264(27):16100-8 PMID: 2777778
  40. The mitochondrial genome of kinetoplastid protozoa: genomic organization, transcription, replication, and evolution.
    Annu Rev Microbiol. 1987;41:363-82 PMID: 2825587
  41. The genes coding for subunit 3 of NADH dehydrogenase and for ribosomal protein S12 are present in the wheat and maize mitochondrial genomes and are co-transcribed.
    Mol Gen Genet. 1988 Dec;215(1):118-27 PMID: 2853827
  42. Specific cleavage of kinetoplast minicircle DNA from Leishmania tarentolae by mung bean nuclease and identification of several additional minicircle sequence classes.
    Nucleic Acids Res. 1986 Jul 11;14(13):5531-56 PMID: 3016649
  43. Kinetoplast DNA in trypanosomid flagellates.
    Int Rev Cytol. 1986;99:119-79 PMID: 3082787
  44. Organization and codon usage of the streptomycin operon in Micrococcus luteus, a bacterium with a high genomic G + C content.
    J Bacteriol. 1987 Oct;169(10):4770-7 PMID: 3654584
  45. Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585-8 PMID: 3856838
  46. Isolation of kinetoplast-mitochondrial complexes from Leishmania tarentolae.
    J Protozool. 1974 Nov;21(5):782-90 PMID: 4217377
  47. Studies on kinetoplast DNA. 3. Kinetic complexity of kinetoplast and nuclear DNA from Leishmania tarentolae.
    Biochim Biophys Acta. 1973 Sep 7;319(3):267-76 PMID: 4747081
  48. The genes for the ribosomal proteins S12 and S7 are clustered with the gene for the EF-Tu protein on the chloroplast genome of Euglena gracilis.
    Nucleic Acids Res. 1984 Mar 26;12(6):2851-9 PMID: 6324129
  49. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.1.56-67.1992
Published
1992-01-00
Pages
56-67
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364069
Subset
IM
Grants
NIAID NIH HHS · AI09102 · United States
NIGMS NIH HHS · GM07104 · United States
Databases
GENBANK
M74225, X56015
External Links
PubMed source DOI: 10.1128/mcb.12.1.56-67.1992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2