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PMID: 6324129 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The genes for the ribosomal proteins S12 and S7 are clustered with the gene for the EF-Tu protein on the chloroplast genome of Euglena gracilis.

Nucleic acids research ·Vol. 12 ·No. 6 ·1984-03-26 ·Pages 2851-9

Montandon PE, Stutz E

Abstract

We characterize a DNA segment of the Euglena gracilis chloroplast DNA fragment Eco . N by nucleotide sequencing and S1 nuclease analysis. We show that this region, which is upstream of the previously sequenced tuf A gene, contains the genes for the ribosomal proteins S12 and S7. The gene arrangement is 5'-rps 12-80 bp spacer-rps 7-174 bp spacer-tuf A, somewhat similar to the str operon of E. coli. The chloroplast S12 and S7 proteins contain 124 and 155 aminoacids, respectively, and are to 68% and 38% homologous with the corresponding E. coli proteins. The region is transcribed into a distronic mRNA of about 1.1 to 1.2 kb. The rps 12 and rps 7 genes, contrary to the tuf A gene, are not split.

MeSH Terms
Amino Acid Sequence Base Sequence Chloroplasts/metabolism DNA/genetics DNA Restriction Enzymes Euglena gracilis/genetics Genes Peptide Elongation Factor Tu Peptide Elongation Factors/genetics Ribosomal Proteins/genetics Transcription, Genetic
Chemicals
Peptide Elongation Factors Ribosomal Proteins ribosomal protein S12 ribosomal protein S7 DNA DNA Restriction Enzymes Peptide Elongation Factor Tu
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Montandon P E
Stutz E
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.6.2851
Published
1984-03-26
Pages
2851-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318710
Subset
IM
Databases
GENBANK
X00480
External Links
PubMed source DOI: 10.1093/nar/12.6.2851 PMC Full Text View journal details
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