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PMID: 8414987 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Trypanosoma brucei minicircles encode multiple guide RNAs which can direct editing of extensively overlapping sequences.

Nucleic acids research ·Vol. 21 ·No. 18 ·1993-09-11 ·Pages 4313-20

Corell RA, Feagin JE, Riley GR, Strickland T, Guderian JA, Myler PJ, Stuart K

Abstract

Small guide RNAs (gRNAs) may direct RNA editing in kinetoplastid mitochondria. We have characterized multiple gRNA genes from Trypanosoma brucei (EATRO 164), that can specify up to 30% of the editing of the COIII, ND7, ND8, and A6 mRNAs and we have also found that the non-translated region of edited COIII mRNA of strain (EATRO 164) differs from that of another strain. Several of the gRNAs specify overlapping regions of the same mRNA often specifying sequence beyond that required for an anchor duplex with the next gRNA. Some gRNAs have different sequence but specify identical editing of the same region of mRNA. These data indicate a complex gRNA population and consequent complex pattern of editing in T. brucei.

Related Genes
COIII Cyb ND7
MeSH Terms
Animals Base Sequence Cloning, Molecular DNA, Circular/genetics DNA, Protozoan/genetics Molecular Sequence Data RNA Editing RNA, Guide/genetics RNA, Messenger/genetics RNA, Protozoan/genetics Sequence Homology, Amino Acid Trypanosoma brucei brucei/genetics
Chemicals
DNA, Circular DNA, Protozoan RNA, Guide RNA, Messenger RNA, Protozoan
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Corell R A
Seattle Biomedical Research Institute, WA 98109-1651.
Feagin J E
Riley G R
Strickland T
Guderian J A
Myler P J
Stuart K
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.18.4313
Published
1993-09-11
Pages
4313-20
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310066
Subset
IM
Grants
NIGMS NIH HHS · 1 F32 GM14126-01A1 · United States
NIAID NIH HHS · AI14102 · United States
NIGMS NIH HHS · GM42188 · United States
Databases
GENBANK
L16535, L16536, L16537, L16538, L16539, L16540, L16541, L16542, L16543, M20379
External Links
PubMed source DOI: 10.1093/nar/21.18.4313 PMC Full Text View journal details
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