Home LiteratureArticle Details
PMID: 8202373 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mitochondrial transcripts are processed but are not edited normally in Trypanosoma equiperdum (ATCC 30019) which has kDNA sequence deletion and duplication.

Nucleic acids research ·Vol. 22 ·No. 9 ·1994-05-11 ·Pages 1696-700

Shu HH, Stuart K

Abstract

Analyses of the Trypanosoma equiperdum (ATCC 30019) maxicircle reveals deletions, duplications and rearrangement compared to T. brucei. The genes for 9S rRNA and 12 proteins are absent. The 12S rRNA and cytochrome oxidase subunit I (COI) genes lack their 3' ends and are adjacent indicating deletion of intervening genes. The remaining two NADH dehydrogenase subunit genes (ND4 and ND5), the ribosomal protein RPS12 gene and the CR5 gene are duplicated and rearranged. ND4, RPS12 and the CR4 transcripts are abundant in steady state RNA while 12S rRNA and COI transcripts are not detected. Full length ND5 transcripts are rare, if present, but chimeric ND5/ND4 transcripts are abundant. The CR4 and RPS12 transcripts are the size of unedited RNAs suggesting that they are processed. However, they are not edited normally, presumably due to the absence of minicircle gRNA genes.

MeSH Terms
Animals DNA, Kinetoplast/genetics DNA, Mitochondrial/genetics DNA, Protozoan Molecular Sequence Data Multigene Family Mutation RNA Editing RNA Processing, Post-Transcriptional RNA, Protozoan Sequence Deletion Transcription, Genetic Trypanosoma/genetics
Chemicals
DNA, Kinetoplast DNA, Mitochondrial DNA, Protozoan RNA, Protozoan
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shu H H
Seattle Biomedical Research Institute, WA 98109.
Stuart K
References (25)
25 references, click to expand
  1. Structural organization of the maxicircle variable region of Trypanosoma brucei: identification of potential replication origins and topoisomerase II binding sites.
    Nucleic Acids Res. 1993 Feb 11;21(3):687-94 PMID: 8382804
  2. The nucleotide sequence of the variable region in Trypanosoma brucei completes the sequence analysis of the maxicircle component of mitochondrial kinetoplast DNA.
    Mol Biochem Parasitol. 1992 Dec;56(2):289-99 PMID: 1336570
  3. Kinetoplast DNA.
    Plasmid. 1979 Jan;2(1):20-40 PMID: 382201
  4. Characterization of the molecular components in kinetoplast-mitochondrial DNA of Trypanosoma equiperdum. Comparative study of the dyskinetoplastic and wild strains.
    J Cell Biol. 1979 Jul;82(1):248-63 PMID: 479299
  5. Restriction cleavage map of kinetoplast DNA minicircles from Trypanosoma equiperdum.
    Biochem Biophys Res Commun. 1979 Sep 27;90(2):405-9 PMID: 508309
  6. Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Short communication.
    Acta Trop. 1979 Sep;36(3):289-92 PMID: 43092
  7. Kinetoplast DNA OF Trypanosoma brucei: physical map of the maxicircle.
    Plasmid. 1979 Oct;2(4):520-8 PMID: 231267
  8. The kinetoplast DNA of Trypanosoma equiperdum.
    Biochim Biophys Acta. 1980 May 30;607(3):397-410 PMID: 6249368
  9. Complete nucleotide sequence of minicircle kinetoplast DNA from Trypanosoma equiperdum.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3323-7 PMID: 6267582
  10. Mapping and 5' end determination of kinetoplast maxicircle gene transcripts from Leishmania tarentolae.
    Nucleic Acids Res. 1985 Aug 26;13(16):5977-93 PMID: 2994021
  11. p-Alkyloxybenzhydroxamic acids, effective inhibitors of the trypanosome glycerol-3-phosphate oxidase.
    Mol Biochem Parasitol. 1986 Jun;19(3):231-40 PMID: 3736594
  12. Diverse patterns of expression of the cytochrome c oxidase subunit I gene and unassigned reading frames 4 and 5 during the life cycle of Trypanosoma brucei.
    Mol Cell Biol. 1985 Nov;5(11):3041-7 PMID: 2427925
  13. Organized packaging of kinetoplast DNA networks.
    Cell. 1986 Nov 21;47(4):537-43 PMID: 3022936
  14. Localization of kinetoplast DNA maxicircle transcripts in bloodstream and procyclic form Trypanosoma brucei.
    Mol Cell Biol. 1982 Jul;2(7):845-52 PMID: 6927793
  15. Extensive editing of the cytochrome c oxidase III transcript in Trypanosoma brucei.
    Cell. 1988 May 6;53(3):413-22 PMID: 2452697
  16. Hypervariability of simple sequences as a general source for polymorphic DNA markers.
    Nucleic Acids Res. 1989 Aug 25;17(16):6463-71 PMID: 2780284
  17. In vivo rearrangement of mitochondrial DNA in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8847-51 PMID: 2682661
  18. A gene required for RNase P activity in Candida (Torulopsis) glabrata mitochondria codes for a 227-nucleotide RNA with homology to bacterial RNase P RNA.
    Mol Cell Biol. 1991 Mar;11(3):1662-7 PMID: 1705011
  19. Trypanosoma equiperdum minicircles encode three distinct primary transcripts which exhibit guide RNA characteristics.
    Mol Cell Biol. 1991 Mar;11(3):1668-75 PMID: 1825348
  20. Modification of Trypanosoma brucei mitochondrial rRNA by posttranscriptional 3' polyuridine tail formation.
    Mol Cell Biol. 1991 Dec;11(12):5878-84 PMID: 1719373
  21. Extensive editing of both processed and preprocessed maxicircle CR6 transcripts in Trypanosoma brucei.
    J Biol Chem. 1992 Jan 15;267(2):1123-8 PMID: 1730639
  22. Maxicircle CR1 transcripts of Trypanosoma brucei are edited and developmentally regulated and encode a putative iron-sulfur protein homologous to an NADH dehydrogenase subunit.
    Mol Cell Biol. 1992 May;12(5):2100-7 PMID: 1373807
  23. Transcript-specific developmental regulation of polyadenylation in Trypanosoma brucei mitochondria.
    Mol Biochem Parasitol. 1992 Jun;52(2):231-40 PMID: 1352374
  24. Mitochondrial DNA of kinetoplastids.
    Int Rev Cytol. 1992;141:65-88 PMID: 1333453
  25. Polymorphism and mitochondrial activity in sleeping sickness trypanosomes.
    Nature. 1965 Nov 20;208(5012):762-6 PMID: 5868887
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.9.1696
Published
1994-05-11
Pages
1696-700
Language
English
Region
England
NLM ID
0411011
PMCID
PMC308051
Subset
IM
Grants
NIAID NIH HHS · AI14102 · United States
Databases
GENBANK
U03738, U03739, U03740, U03741
External Links
PubMed source DOI: 10.1093/nar/22.9.1696 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2