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PMID: 8382804 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural organization of the maxicircle variable region of Trypanosoma brucei: identification of potential replication origins and topoisomerase II binding sites.

Nucleic acids research ·Vol. 21 ·No. 3 ·1993-02-11 ·Pages 687-94

Myler PJ, Glick D, Feagin JE, Morales TH, Stuart KD

Abstract

The maxicircle of the parasitic protozoan Trypanosoma brucei, one component of the mitochondrial genome, has size differences among isolates that localize to the variable region (VR) between the ND5 and 12S rRNA genes. We present here the nucleotide sequence of this entire region, thus completing the sequence of the maxicircle genome. We also find heterogeneously sized transcripts from throughout most of the VR. The VR has three distinct sections, each with characteristic repeated sequences. The repeated sequences in two sections are short and highly reiterated; the intraspecies size variation occurs within this region. The third section contains non-repetitive sequences and a large duplication immediately upstream of the 12S rRNA gene. Two repeat units within section I contain a sequence that has homology to the DNA replication origin of minicircles. This region also contains sequences with homology to topoisomerase II binding and cleavage sites. These findings suggest a role for the VR in DNA replication of the maxicircle.

MeSH Terms
Animals Base Sequence Binding Sites Blotting, Northern DNA Replication DNA Topoisomerases, Type II/metabolism DNA, Circular/biosynthesis,chemistry,genetics DNA, Protozoan/biosynthesis,chemistry,genetics Genetic Variation Molecular Sequence Data Repetitive Sequences, Nucleic Acid Restriction Mapping Trypanosoma brucei brucei/genetics
Chemicals
DNA, Circular DNA, Protozoan DNA Topoisomerases, Type II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Myler P J
Seattle Biomedical Research Institute, WA 98109-1651.
Glick D
Feagin J E
Morales T H
Stuart K D
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.3.687
Published
1993-02-11
Pages
687-94
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309170
Subset
IM
Grants
NIAID NIH HHS · AI14102 · United States
Databases
GENBANK
Z15118
External Links
PubMed source DOI: 10.1093/nar/21.3.687 PMC Full Text View journal details
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