Home LiteratureArticle Details
PMID: 2437529 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RNA end-labeling and RNA ligase activities can produce a circular rRNA in whole cell extracts from trypanosomes.

Nucleic acids research ·Vol. 15 ·No. 8 ·1987-04-24 ·Pages 3275-90

White TC, Borst P

Abstract

We have found two enzymatic activities in whole cell extracts from Trypanosoma brucei; an end-labeling reaction involving a single uridine at the 3' end of ribosomal RNAs (rRNAs) and an RNA ligase activity joining 5' monophosphates to 3' hydroxyl groups. The RNA ligase acts upon one of the small rRNAs (180 nucleotides) from the trypanosome ribosomal repeat unit, forming a circular RNA. The specific circularization of this small rRNA is probably dependent on the secondary structure of the molecule and is not detectable in vivo.

MeSH Terms
Animals Isotope Labeling/methods Nucleic Acid Conformation Polynucleotide Ligases/metabolism RNA/biosynthesis RNA Ligase (ATP)/metabolism RNA Nucleotidyltransferases/metabolism RNA, Circular RNA, Ribosomal/metabolism Trypanosoma brucei brucei/enzymology,genetics Uridine Triphosphate/metabolism
Chemicals
RNA, Circular RNA, Ribosomal RNA RNA Nucleotidyltransferases UTP-RNA uridylyltransferase Polynucleotide Ligases RNA Ligase (ATP) Uridine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
White T C
Borst P
References (30)
30 references, click to expand
  1. Chain extension of ribonucleic acid by enzymes from rat liver cytoplasm.
    Biochem J. 1968 Oct;109(4):485-94 PMID: 5683501
  2. Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.
    Exp Parasitol. 1970 Dec;28(3):521-34 PMID: 4993889
  3. A poly(U) polymerase in tobacco leaves.
    Biochim Biophys Acta. 1975 Apr 2;383(4):351-8 PMID: 235994
  4. Poly(U) polymerase in rat brain.
    Nature. 1975 Jul 24;256(5515):337-9 PMID: 1143338
  5. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  6. Association of poly(A) and poly(U) polymerases with cytoplasmic ribosomes.
    Eur J Biochem. 1978 Mar;84(1):113-21 PMID: 565709
  7. Reactions at the termini of tRNA with T4 RNA ligase.
    Nucleic Acids Res. 1978 Oct;5(10):3665-77 PMID: 724498
  8. Comparison of endogenous and exogenous RNA primers of poly(U) polymerase in rat hepatic ribosomes.
    Biochem J. 1979 Mar 1;177(3):895-902 PMID: 444210
  9. Direct chemical method for sequencing RNA.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760-4 PMID: 377283
  10. Distinctive RNA transcriptase, polyadenylic acid polymerase, and polyuridylic acid polymerase activities associated with Pichinde virus.
    J Virol. 1979 Apr;30(1):98-107 PMID: 225533
  11. Splicing of yeast tRNA precursors: structure of the reaction intermediates.
    Cell. 1979 Sep;18(1):37-45 PMID: 389432
  12. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  13. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
    Eur J Biochem. 1980 Jun;107(2):303-14 PMID: 6772444
  14. Isolation of a foot-and-mouth disease polyuridylic acid polymerase and its inhibition by antibody.
    J Virol. 1980 Feb;33(2):774-9 PMID: 6251248
  15. RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in the spliced transfer RNA.
    J Mol Biol. 1981 Jan 15;145(2):405-20 PMID: 7196457
  16. Terminal uridylyl transferase of Vigna unguiculata: purification and characterization of an enzyme catalyzing the addition of a single UMP residue to the 3'-end of an RNA primer.
    Nucleic Acids Res. 1981 Jun 11;9(11):2433-53 PMID: 6269049
  17. The structure of the yeast ribosomal RNA genes. 4. Complete sequence of the 25 S rRNA gene from Saccharomyces cerevisae.
    Nucleic Acids Res. 1981 Dec 21;9(24):6953-8 PMID: 6460984
  18. RNA ligation via 2'-phosphomonoester, 3'5'-phosphodiester linkage: requirement of 2',3'-cyclic phosphate termini and involvement of a 5'-hydroxyl polynucleotide kinase.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1474-8 PMID: 6280184
  19. Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.
    Nucleic Acids Res. 1982 Dec 11;10(23):7521-9 PMID: 6760127
  20. RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa cell extract.
    Nucleic Acids Res. 1983 Mar 11;11(5):1405-18 PMID: 6828385
  21. Primary structures of four novel small ribosomal RNAs from Crithidia fasciculata.
    Can J Biochem Cell Biol. 1983 Jan;61(1):38-45 PMID: 6850409
  22. Activation of the genes for variant surface glycoproteins 117 and 118 in Trypanosoma brucei.
    J Mol Biol. 1983 Jun 5;166(4):537-56 PMID: 6306248
  23. Discontinuous synthesis of mRNA in trypanosomes.
    EMBO J. 1984 Oct;3(10):2387-92 PMID: 6094183
  24. Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.
    Nucleic Acids Res. 1984 Nov 26;12(22):8639-51 PMID: 6095205
  25. Alpha-amanitin-insensitive transcription of variant surface glycoprotein genes provides further evidence for discontinuous transcription in trypanosomes.
    Nucleic Acids Res. 1984 Dec 21;12(24):9457-72 PMID: 6514580
  26. Poliovirus replicase stimulation by terminal uridylyl transferase.
    J Biol Chem. 1985 Jun 25;260(12):7628-35 PMID: 2987262
  27. The 5.8S ribosomal RNA gene of Trypanosoma brucei: structural and transcriptional studies.
    Nucleic Acids Res. 1985 May 24;13(10):3533-49 PMID: 4011434
  28. Purification of a terminal uridylyltransferase that acts as host factor in the in vitro poliovirus replicase reaction.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):221-5 PMID: 2417240
  29. Excised group II introns in yeast mitochondria are lariats and can be formed by self-splicing in vitro.
    Cell. 1986 Jan 31;44(2):225-34 PMID: 2417726
  30. Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to domain VII of other eukaryotic 28S rRNAs.
    Nucleic Acids Res. 1986 Dec 9;14(23):9471-89 PMID: 3797245
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.8.3275
Published
1987-04-24
Pages
3275-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340729
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.8.3275 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2