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PMID: 6760127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.

Nucleic acids research ·Vol. 10 ·No. 23 ·1982-12-11 ·Pages 7521-9

Kikuchi Y, Tyc K, Filipowicz W, Sänger HL, Gross HJ

Abstract

A novel type of RNA ligase activity in extracts of wheat germ or Chlamydomonas requires 2', 3'-cyclic phosphate and 5'-phosphate ends for ligation to form a 2'-phosphomonoester, 3',5'-phosphodiester bond. Using 5'-3 2P-labeled linear PSTV, we demonstrate that RNase T1-nicked viroid predominantly forms (formula; see text) U-bonds. Natural linear PSTV, however, forms mainly (formula; see text) A-bonds upon enzymatic circularization. We show that natural linear PSTV RNA has nicks between C181 and A182, or between C348 and A349, and that consequently C181 and C348 carry 2',3'-cyclophosphate termini.

MeSH Terms

Chemical Phenomena Chemistry Chlamydomonas/enzymology Phosphorus Radioisotopes Plants/enzymology Polynucleotide Ligases/metabolism RNA Ligase (ATP)/metabolism RNA, Viral Radioisotope Dilution Technique Triticum/enzymology Viroids/analysis

Chemicals

Phosphorus Radioisotopes RNA, Viral Polynucleotide Ligases RNA Ligase (ATP)

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Kikuchi Y

Tyc K

Filipowicz W

Sänger H L

Gross H J

References (10)

10 references, click to expand

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Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs.
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Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.
Methods Enzymol. 1979;59:58-109 PMID: 220499
Cell-free circularization of viroid progeny RNA by an RNA ligase from wheat germ.
Science. 1982 Sep 17;217(4565):1147-9 PMID: 17740972
Nucleotide sequence and secondary structure of potato spindle tuber viroid.
Nature. 1978 May 18;273(5659):203-8 PMID: 643081
Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.
Nature. 1981 Sep 10-16;293(5828):112-6 PMID: 7266664
RNA ligation via 2'-phosphomonoester, 3'5'-phosphodiester linkage: requirement of 2',3'-cyclic phosphate termini and involvement of a 5'-hydroxyl polynucleotide kinase.
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1474-8 PMID: 6280184
Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures.
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3852-6 PMID: 1069269
Replicative form of Semliki Forest virus RNA contains an unpaired guanosine.
Nature. 1979 Dec 13;282(5740):754-6 PMID: 514358
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.23.7521

Published

1982-12-11

Pages

7521-9

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327027

Subset

External Links

PubMed source DOI: 10.1093/nar/10.23.7521 PMC Full Text View journal details

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Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.

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