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PMID: 8879408 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Metabolic engineering of sugar catabolism in lactic acid bacteria.

Antonie van Leeuwenhoek ·Vol. 70 ·No. 2-4 ·1996-10-00 ·Pages 223-42

de Vos WM

Abstract

Lactic acid bacteria are characterized by a relatively simple sugar fermentation pathway that, by definition, results in the formation of lactic acid. The extensive knowledge of traditional pathways and the accumulating genetic information on these and novel ones, allows for the rerouting of metabolic processes in lactic acid bacteria by physiological approaches, genetic methods, or a combination of these two. This review will discuss past and present examples and future possibilities of metabolic engineering of lactic acid bacteria for the production of important compounds, including lactic and other acids, flavor compounds, and exopolysaccharides.

MeSH Terms
Biological Transport Carbohydrate Metabolism Fermentation Genetic Engineering Lactic Acid/metabolism Lactobacillus/enzymology,genetics,metabolism Oxidation-Reduction Oxidoreductases/metabolism Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism Polysaccharides, Bacterial/biosynthesis Pyruvic Acid/metabolism Streptococcaceae/enzymology,genetics,metabolism
Chemicals
Polysaccharides, Bacterial Lactic Acid Pyruvic Acid Oxidoreductases Phosphoenolpyruvate Sugar Phosphotransferase System
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
de Vos W M
Department of Biophysical Chemistry, NIZO, Ede, The Netherlands.
References (102)
102 references, click to expand
  1. Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis.
    J Bacteriol. 1993 Jul;175(14):4383-90 PMID: 8331070
  2. Characterization of the Lactococcus lactis lactose operon promoter: contribution of flanking sequences and LacR repressor to promoter activity.
    J Bacteriol. 1992 Apr;174(7):2273-80 PMID: 1372602
  3. Altered metabolism in a Streptococcus lactis C2 mutant deficient in lactic dehydrogenase.
    J Dairy Sci. 1974 Feb;57(2):181-6 PMID: 4211787
  4. Regulation of bacterial sugar-H+ symport by phosphoenolpyruvate-dependent enzyme I/HPr-mediated phosphorylation.
    Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):778-82 PMID: 7846050
  5. Uncommon pathways of metabolism among lactic acid bacteria.
    FEMS Microbiol Rev. 1990 Sep;7(1-2):103-11 PMID: 2271221
  6. Cloning and expression of the manganese superoxide dismutase gene of Escherichia coli in Lactococcus lactis and Lactobacillus gasseri.
    Mol Gen Genet. 1993 May;239(1-2):33-40 PMID: 8510661
  7. Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and alpha-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis.
    J Bacteriol. 1992 Jul;174(14):4838-41 PMID: 1624471
  8. Molecular cloning and DNA sequence of lacE, the gene encoding the lactose-specific enzyme II of the phosphotransferase system of Lactobacillus casei. Evidence that a cysteine residue is essential for sugar phosphorylation.
    J Biol Chem. 1990 Dec 25;265(36):22561-8 PMID: 2125053
  9. Bacterial exopolysaccharides.
    Adv Microb Physiol. 1972;8:143-213 PMID: 4581483
  10. Structure and expression of the Lactococcus lactis gene for phospho-beta-galactosidase (lacG) in Escherichia coli and L. lactis.
    J Gen Microbiol. 1989 Jul;135(7):1833-46 PMID: 2515252
  11. Autoregulation of nisin biosynthesis in Lactococcus lactis by signal transduction.
    J Biol Chem. 1995 Nov 10;270(45):27299-304 PMID: 7592991
  12. Nucleotide and deduced amino acid sequences of the lacR, lacABCD, and lacFE genes encoding the repressor, tagatose 6-phosphate gene cluster, and sugar-specific phosphotransferase system components of the lactose operon of Streptococcus mutans.
    J Bacteriol. 1992 Oct;174(19):6159-70 PMID: 1400164
  13. Characterization of two cold-sensitive mutants of the beta-galactosidase from Lactobacillus delbruckii subsp. bulgaricus.
    J Biol Chem. 1994 Feb 25;269(8):5666-72 PMID: 8119903
  14. Inhibition of the phosphoenolpyruvate:lactose phosphotransferase system and activation of a cytoplasmic sugar-phosphate phosphatase in Lactococcus lactis by ATP-dependent metabolite-activated phosphorylation of serine 46 in the phosphocarrier protein HPr.
    J Biol Chem. 1994 Apr 22;269(16):11837-44 PMID: 8163482
  15. Molecular cloning and analysis of the gene encoding the NADH oxidase from Streptococcus faecalis 10C1. Comparison with NADH peroxidase and the flavoprotein disulfide reductases.
    J Mol Biol. 1992 Oct 5;227(3):658-71 PMID: 1404382
  16. Cloning, sequence and overexpression of NADH peroxidase from Streptococcus faecalis 10C1. Structural relationship with the flavoprotein disulfide reductases.
    J Mol Biol. 1991 Oct 5;221(3):857-71 PMID: 1719212
  17. Carbohydrate utilization in Streptococcus thermophilus: characterization of the genes for aldose 1-epimerase (mutarotase) and UDPglucose 4-epimerase.
    J Bacteriol. 1990 Jul;172(7):4037-47 PMID: 1694527
  18. Cloning, sequencing and expression in Escherichia coli of the ptsI gene encoding enzyme I of the phosphoenolpyruvate:sugar phosphotransferase transport system from Streptococcus salivarius.
    Gene. 1992 Nov 2;121(1):71-8 PMID: 1427100
  19. Molecular cloning and sequence analysis of the gene encoding the H2O2-forming NADH oxidase from Streptococcus mutans.
    Biosci Biotechnol Biochem. 1994 Sep;58(9):1603-7 PMID: 7765479
  20. Cloning and sequence analysis of the gene encoding L-lactate dehydrogenase from Lactococcus lactis: evolutionary relationships between 21 different LDH enzymes.
    Gene. 1992 Dec 1;122(1):193-7 PMID: 1452029
  21. Lactobacillus plantarum ldhL gene: overexpression and deletion.
    J Bacteriol. 1994 Feb;176(3):596-601 PMID: 8300514
  22. Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase.
    J Bacteriol. 1993 May;175(9):2541-51 PMID: 8478320
  23. Generation of an electrochemical proton gradient in Streptococcus cremoris by lactate efflux.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5502-6 PMID: 6254084
  24. Sequence and characteristics of the Bifidobacterium longum gene encoding L-lactate dehydrogenase and the primary structure of the enzyme: a new feature of the allosteric site.
    Gene. 1989 Dec 21;85(1):161-8 PMID: 2695396
  25. Iron- and manganese-containing superoxide dismutases can be distinguished by analysis of their primary structures.
    FEBS Lett. 1988 Mar 14;229(2):377-82 PMID: 3345848
  26. Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria.
    Microbiol Rev. 1993 Sep;57(3):543-94 PMID: 8246840
  27. Identical transcriptional control of the divergently transcribed prtP and prtM genes that are required for proteinase production in lactococcus lactis SK11.
    J Bacteriol. 1996 Mar;178(6):1525-31 PMID: 8626277
  28. Occurrence of glutathione in bacteria.
    J Bacteriol. 1978 Mar;133(3):1126-9 PMID: 417060
  29. Exocellular polysaccharides produced by lactic acid bacteria.
    FEMS Microbiol Rev. 1990 Sep;7(1-2):113-30 PMID: 1702979
  30. Analysis of the lacZ sequences from two Streptococcus thermophilus strains: comparison with the Escherichia coli and Lactobacillus bulgaricus beta-galactosidase sequences.
    J Gen Microbiol. 1991 Feb;137(2):369-80 PMID: 1901904
  31. Cooperative binding of lactose and the phosphorylated phosphocarrier protein HPr(Ser-P) to the lactose/H+ symport permease of Lactobacillus brevis.
    Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):417-21 PMID: 7831302
  32. Regulation of the multiple sugar metabolism operon in Streptococcus mutans.
    Dev Biol Stand. 1995;85:343-50 PMID: 8586199
  33. The lactose transporter in Leuconostoc lactis is a new member of the LacS subfamily of galactoside-pentose-hexuronide translocators.
    Appl Environ Microbiol. 1996 May;62(5):1574-82 PMID: 8633855
  34. Manganese, superoxide dismutase, and oxygen tolerance in some lactic acid bacteria.
    J Bacteriol. 1981 Jun;146(3):928-36 PMID: 6263860
  35. Stress response in Lactococcus lactis: cloning, expression analysis, and mutation of the lactococcal superoxide dismutase gene.
    J Bacteriol. 1995 Sep;177(18):5254-60 PMID: 7665513
  36. The sum of the control coefficients of all enzymes on the flux through a group-transfer pathway can be as high as two.
    Eur J Biochem. 1993 Mar 15;212(3):791-9 PMID: 8462550
  37. Characterization of the gor gene of the lactic acid bacterium Streptococcus thermophilus CNRZ368.
    Res Microbiol. 1995 Jun;146(5):371-83 PMID: 8525054
  38. Expression and nucleotide sequence of the Lactobacillus bulgaricus beta-galactosidase gene cloned in Escherichia coli.
    J Bacteriol. 1989 Feb;171(2):625-35 PMID: 2492511
  39. Lactose metabolism in Streptococcus lactis: studies with a mutant lacking glucokinase and mannose-phosphotransferase activities.
    J Bacteriol. 1985 Apr;162(1):217-23 PMID: 3920203
  40. Structure of NADH peroxidase from Streptococcus faecalis 10C1 refined at 2.16 A resolution.
    J Mol Biol. 1991 Oct 20;221(4):1325-44 PMID: 1942054
  41. Control analysis of the dependence of Escherichia coli physiology on the H(+)-ATPase.
    Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8068-72 PMID: 8367465
  42. Galactokinase activity in Streptococcus thermophilus.
    Appl Environ Microbiol. 1985 Oct;50(4):777-80 PMID: 4083880
  43. A system to generate chromosomal mutations in Lactococcus lactis which allows fast analysis of targeted genes.
    J Bacteriol. 1995 Dec;177(24):7011-8 PMID: 8522504
  44. Properties of a Streptococcus lactis strain that ferments lactose slowly.
    J Bacteriol. 1984 Jan;157(1):28-34 PMID: 6418719
  45. Cloning, sequencing and expression of the L-2-hydroxyisocaproate dehydrogenase-encoding gene of Lactobacillus confusus in Escherichia coli.
    Gene. 1989 Nov 30;83(2):263-70 PMID: 2684788
  46. L-(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans.
    J Bacteriol. 1994 Mar;176(5):1542-5 PMID: 8113201
  47. Lysines 72, 80 and 213 and aspartic acid 210 of the Lactococcus lactis LacR repressor are involved in the response to the inducer tagatose-6-phosphate leading to induction of lac operon expression.
    Protein Eng. 1993 Feb;6(2):201-6 PMID: 8475045
  48. Immunological crossreactivity to the catabolite control protein CcpA Bacillus megaterium is found in many gram-positive bacteria.
    FEMS Microbiol Lett. 1996 Jun 1;139(2-3):109-15 PMID: 8674978
  49. Identification and characterization of the eps (Exopolysaccharide) gene cluster from Streptococcus thermophilus Sfi6.
    J Bacteriol. 1996 Mar;178(6):1680-90 PMID: 8626297
  50. Cloning, sequencing and expression in Escherichia coli of the D-2-hydroxyisocaproate dehydrogenase gene of Lactobacillus casei.
    Gene. 1989 May 15;78(1):47-57 PMID: 2504649
  51. Cloning and characterization of the scrA gene encoding the sucrose-specific Enzyme II of the phosphotransferase system from Staphylococcus xylosus.
    Mol Gen Genet. 1993 Oct;241(1-2):33-41 PMID: 8232209
  52. Molecular cloning and sequence analysis of the gene encoding the H2O-forming NADH oxidase from Streptococcus mutans.
    Biosci Biotechnol Biochem. 1996 Jan;60(1):39-43 PMID: 8824824
  53. Identification of an anaerobically induced phosphoenolpyruvate-dependent fructose-specific phosphotransferase system and evidence for the Embden-Meyerhof glycolytic pathway in the heterofermentative bacterium Lactobacillus brevis.
    J Bacteriol. 1996 Jan;178(1):314-6 PMID: 8550437
  54. Cloning and nucleotide sequence of the Lactobacillus casei lactate dehydrogenase gene.
    Appl Environ Microbiol. 1991 Aug;57(8):2413-7 PMID: 1768113
  55. Energy transduction in lactic acid bacteria.
    FEMS Microbiol Rev. 1993 Sep;12(1-3):125-47 PMID: 8398212
  56. Effect of oxygen on lactose metabolism in lactic streptococci.
    Appl Environ Microbiol. 1987 Mar;53(3):533-41 PMID: 16347303
  57. Homologous integration and transposition to identify genes involved in the production of exopolysaccharides in Streptococcus thermophilus.
    Dev Biol Stand. 1995;85:487-93 PMID: 8586221
  58. Genetics of the nisin operon and the sucrose-nisin conjugative transposon Tn5276.
    Dev Biol Stand. 1995;85:617-25 PMID: 8586240
  59. Molecular cloning, transcriptional analysis, and nucleotide sequence of lacR, a gene encoding the repressor of the lactose phosphotransferase system of Lactococcus lactis.
    J Biol Chem. 1990 Oct 25;265(30):18499-503 PMID: 2120234
  60. Cloning, nucleotide sequence, expression, and chromosomal location of ldh, the gene encoding L-(+)-lactate dehydrogenase, from Lactococcus lactis.
    J Bacteriol. 1992 Nov;174(21):6956-64 PMID: 1400245
  61. Structure of the extracellular polysaccharide from slime-forming Lactococcus lactis subsp. cremoris SBT 0495.
    Carbohydr Res. 1992 Feb 7;224:245-53 PMID: 1591765
  62. Nucleotide sequence of Streptococcus mutans superoxide dismutase gene and isolation of insertion mutants.
    J Bacteriol. 1992 Aug;174(15):4928-34 PMID: 1321118
  63. Genetics of lactose utilization in lactic acid bacteria.
    FEMS Microbiol Rev. 1994 Oct;15(2-3):217-37 PMID: 7946468
  64. Stimulation of dihydroxyacetone and glycerol kinase activity in Streptococcus faecalis by phosphoenolpyruvate-dependent phosphorylation catalyzed by enzyme I and HPr of the phosphotransferase system.
    J Bacteriol. 1986 Jun;166(3):829-36 PMID: 3011747
  65. Evidence that L-(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans.
    Infect Immun. 1994 Jan;62(1):60-4 PMID: 8262650
  66. DNA sequence and in vitro mutagenesis of the gene encoding the fructose-1,6-diphosphate-dependent L-(+)-lactate dehydrogenase of Streptococcus mutans.
    Infect Immun. 1991 Nov;59(11):3930-4 PMID: 1937751
  67. Phosphotransferase system of Streptococcus salivarius: characterization of the ptsH gene and its product.
    Gene. 1993 Dec 22;136(1-2):27-34 PMID: 8294015
  68. Promoter analysis and transcriptional regulation of Lactobacillus pentosus genes involved in xylose catabolism.
    Mol Gen Genet. 1994 Oct 17;245(1):117-25 PMID: 7845354
  69. ABC transporters: bacterial exporters.
    Microbiol Rev. 1993 Dec;57(4):995-1017 PMID: 8302219
  70. Mechanistic and physiological consequences of HPr(ser) phosphorylation on the activities of the phosphoenolpyruvate:sugar phosphotransferase system in gram-positive bacteria: studies with site-specific mutants of HPr.
    EMBO J. 1989 Jul;8(7):2111-20 PMID: 2507315
  71. Lactose metabolism in Lactobacillus bulgaricus: analysis of the primary structure and expression of the genes involved.
    J Bacteriol. 1991 Mar;173(6):1951-7 PMID: 1705929
  72. A new mobile genetic element in Lactobacillus delbrueckii subsp. bulgaricus.
    Mol Gen Genet. 1995 Aug 30;248(4):407-16 PMID: 7565604
  73. Arginine transport in Streptococcus lactis is catalyzed by a cationic exchanger.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6093-7 PMID: 2819865
  74. Cloning, sequence, and phenotypic expression of katA, which encodes the catalase of Lactobacillus sake LTH677.
    Appl Environ Microbiol. 1992 Mar;58(3):832-9 PMID: 1575485
  75. Purification and analysis of streptococcal NADH peroxidase expressed in Escherichia coli.
    J Biol Chem. 1993 Feb 15;268(5):3161-7 PMID: 8428993
  76. Molecular cloning, characterization, and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis.
    J Biol Chem. 1991 Apr 15;266(11):7176-81 PMID: 1901863
  77. Growth and Energy Generation by Lactococcus lactis subsp. lactis biovar diacetylactis during Citrate Metabolism.
    Appl Environ Microbiol. 1993 Dec;59(12):4216-22 PMID: 16349120
  78. Regulation of sugar uptake and efflux in gram-positive bacteria.
    FEMS Microbiol Rev. 1989 Jun;5(1-2):149-56 PMID: 2699246
  79. Functional analysis of promoters in the nisin gene cluster of Lactococcus lactis.
    J Bacteriol. 1996 Jun;178(12):3434-9 PMID: 8655538
  80. Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis.
    J Biol Chem. 1990 Dec 25;265(36):22554-60 PMID: 2125052
  81. Protein kinase-dependent HPr/CcpA interaction links glycolytic activity to carbon catabolite repression in gram-positive bacteria.
    Mol Microbiol. 1995 Mar;15(6):1049-53 PMID: 7623661
  82. Sequence and expression of the genes for HPr (ptsH) and enzyme I (ptsI) of the phosphoenolpyruvate-dependent phosphotransferase transport system from Streptococcus mutans.
    Infect Immun. 1994 Apr;62(4):1156-65 PMID: 8132321
  83. A binding protein-dependent transport system in Streptococcus mutans responsible for multiple sugar metabolism.
    J Biol Chem. 1992 Mar 5;267(7):4631-7 PMID: 1537846
  84. Catabolite repression in Bacillus subtilis: a global regulatory mechanism for the gram-positive bacteria?
    Mol Microbiol. 1995 Feb;15(3):395-401 PMID: 7540244
  85. Metabolic engineering of Lactococcus lactis: influence of the overproduction of alpha-acetolactate synthase in strains deficient in lactate dehydrogenase as a function of culture conditions.
    Appl Environ Microbiol. 1995 Nov;61(11):3967-71 PMID: 8526510
  86. Characterization of pepR1, a gene coding for a potential transcriptional regulator of Lactobacillus delbrueckii subsp. lactis DSM7290.
    FEMS Microbiol Lett. 1996 Feb 1;136(1):63-9 PMID: 8919457
  87. Spontaneous deletion formation within the beta-galactosidase gene of Lactobacillus bulgaricus.
    J Bacteriol. 1990 Oct;172(10):5670-6 PMID: 2120187
  88. Structure of an exocellular polysaccharide produced by Streptococcus thermophilus.
    Carbohydr Res. 1990 May 1;198(2):313-21 PMID: 2165858
  89. Dependence of lactose metabolism upon mutarotase encoded in the gal operon in Escherichia coli.
    J Mol Biol. 1994 Dec 2;244(3):269-78 PMID: 7966338
  90. Characterization and sequence analysis of the scrA gene encoding enzyme IIScr of the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system.
    J Bacteriol. 1989 Jan;171(1):263-71 PMID: 2536656
  91. Selection of Galactose-Fermenting Streptococcus thermophilus in Lactose-Limited Chemostat Cultures.
    Appl Environ Microbiol. 1984 Jul;48(1):186-91 PMID: 16346586
  92. Identification and characterization of the alpha-acetolactate synthase gene from Lactococcus lactis subsp. lactis biovar diacetylactis.
    Appl Environ Microbiol. 1994 Apr;60(4):1390-4 PMID: 8017926
  93. Cloning and overexpression of the Lactobacillus bulgaricus NAD(+)-dependent D-lactate dehydrogenase gene in Escherichia coli:purification and characterization of the recombinant enzyme.
    Biochem Biophys Res Commun. 1992 Jun 15;185(2):705-12 PMID: 1610363
  94. Proton motive force generation by citrolactic fermentation in Leuconostoc mesenteroides.
    J Bacteriol. 1996 Apr;178(8):2178-85 PMID: 8636016
  95. Lactose transport system of Streptococcus thermophilus: a hybrid protein with homology to the melibiose carrier and enzyme III of phosphoenolpyruvate-dependent phosphotransferase systems.
    J Bacteriol. 1989 Jan;171(1):244-53 PMID: 2644191
  96. Cation and sugar selectivity determinants in a novel family of transport proteins.
    Mol Microbiol. 1996 Mar;19(5):911-22 PMID: 8830272
  97. NAD(+)-dependent D-2-hydroxyisocaproate dehydrogenase of Lactobacillus delbrueckii subsp. bulgaricus. Gene cloning and enzyme characterization.
    Eur J Biochem. 1994 Sep 1;224(2):439-46 PMID: 7925358
  98. D-lactate dehydrogenase is a member of the D-isomer-specific 2-hydroxyacid dehydrogenase family. Cloning, sequencing, and expression in Escherichia coli of the D-lactate dehydrogenase gene of Lactobacillus plantarum.
    J Biol Chem. 1991 Jul 5;266(19):12588-94 PMID: 1840590
  99. Cloning, nucleotide sequence, and transcriptional analysis of the Pediococcus acidilactici L-(+)-lactate dehydrogenase gene.
    Appl Environ Microbiol. 1995 Jan;61(1):266-72 PMID: 7887607
  100. A beta-galactosidase deletion mutant of Lactobacillus bulgaricus reverts to generate an active enzyme by internal DNA sequence duplication.
    Mol Gen Genet. 1991 May;227(1):17-21 PMID: 1904535
  101. Characterization of the novel nisin-sucrose conjugative transposon Tn5276 and its insertion in Lactococcus lactis.
    J Bacteriol. 1992 Feb;174(4):1280-7 PMID: 1310502
  102. In Vivo Cloning of lac Genes in Streptococcus lactis ML3.
    Appl Environ Microbiol. 1984 Feb;47(2):245-9 PMID: 16346463
Article Info
Journal
Antonie van Leeuwenhoek
Abbr.
Antonie Van Leeuwenhoek
ISSN
0003-6072
DOI
10.1007/BF00395934
Published
1996-10-00
Pages
223-42
Language
English
Region
Netherlands
NLM ID
0372625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00395934 View journal details
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