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PMID: 7887607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning, nucleotide sequence, and transcriptional analysis of the Pediococcus acidilactici L-(+)-lactate dehydrogenase gene.

Applied and environmental microbiology ·Vol. 61 ·No. 1 ·1995-01-00 ·Pages 266-72

Garmyn D, Ferain T, Bernard N, Hols P, Delcour J

Abstract

Recombinant plasmids containing the Pediococcus acidilactici L-(+)-lactate dehydrogenase gene (ldhL) were isolated by complementing for growth under anaerobiosis of an Escherichia coli lactate dehydrogenase-pyruvate formate lyase double mutant. The nucleotide sequence of the ldhL gene predicted a protein of 323 amino acids showing significant similarity with other bacterial L-(+)-lactate dehydrogenases and especially with that of Lactobacillus plantarum. The ldhL transcription start points in P. acidilactici were defined by primer extension, and the promoter sequence was identified as TCAAT-(17 bp)-TATAAT. This sequence is closely related to the consensus sequence of vegetative promoters from gram-positive bacteria as well as from E. coli. Northern analysis of P. acidilactici RNA showed a 1.1-kb ldhL transcript whose abundance is growth rate regulated. These data, together with the presence of a putative rho-independent transcriptional terminator, suggest that ldhL is expressed as a monocistronic transcript in P. acidilactici.

Related Genes
ldhZ
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Cloning, Molecular Conserved Sequence Genes, Bacterial L-Lactate Dehydrogenase/genetics Lactobacillus/enzymology,genetics Molecular Sequence Data Pediococcus/enzymology,genetics Sequence Alignment Transcription, Genetic
Chemicals
L-Lactate Dehydrogenase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Garmyn D
Laboratoire de Génétique Moléculaire, Université Catholique de Louvain, Belgium.
Ferain T
Bernard N
Hols P
Delcour J
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.61.1.266-272.1995
Published
1995-01-00
Pages
266-72
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC167282
Subset
IM
Databases
GENBANK
X70927
External Links
PubMed source DOI: 10.1128/aem.61.1.266-272.1995 PMC Full Text View journal details
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