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PMID: 2202593 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cleavages in the 5' region of the ompA and bla mRNA control stability: studies with an E. coli mutant altering mRNA stability and a novel endoribonuclease.

The EMBO journal ·Vol. 9 ·No. 9 ·1990-09-00 ·Pages 2731-41

Lundberg U, von Gabain A, Melefors O

Abstract

We describe here the partial purification of a novel Escherichia coli endoribonuclease, RNase K. This protein catalyses site-specific cleavages in the 5' region of in vitro transcribed ompA and bla transcripts. Some of the resulting cleavage products are also found in cellular ompA mRNA, defining the in vivo activity of RNase K. The following evidence suggests that RNase K initiates mRNA degradation. First, RNase K cleavages are suppressed in the ams mutant, which has a generally prolonged mRNA half-life. Secondly, RNase K cleavage products seem to have very short half-lives in vivo, indicating that they are decay intermediates rather than processing products. Thirdly, the differences in in vivo half-life between the ompA and bla mRNAs are mimicked in in vitro decay reactions with purified RNase K. The relationship between RNase K and the ams locus might point to a more general role of RNase K in mRNA degradation. We discuss the influence of mRNA secondary structure on RNase K cleavage specificity.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics Base Sequence Endoribonucleases/isolation & purification,metabolism Escherichia coli/enzymology,genetics Models, Molecular Molecular Sequence Data Mutation Nucleic Acid Conformation Oligonucleotide Probes Plasmids RNA, Messenger/genetics,metabolism Transcription, Genetic beta-Lactamases/genetics
Chemicals
Bacterial Outer Membrane Proteins Oligonucleotide Probes RNA, Messenger Endoribonucleases ribonuclease K beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lundberg U
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden.
von Gabain A
Melefors O
References (53)
53 references, click to expand
  1. Purification and properties of ribonuclease III from Escherichia coli.
    J Biol Chem. 1968 Jan 10;243(1):82-91 PMID: 4865702
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Differential stability of trp messenger RNA synthesized originating at the trp promoter and pL promoter of lambda trp phage.
    J Mol Biol. 1975 Feb 25;92(2):289-304 PMID: 1142425
  4. Characterization of an endoribonuclease, RNase N, from Escherichia coli.
    J Biol Chem. 1978 Aug 25;253(16):5594-9 PMID: 97300
  5. A conditional lethal mutation in an Escherichia coli strain with a longer chemical lifetime of messenger RNA.
    J Mol Biol. 1979 Apr 15;129(3):343-57 PMID: 110942
  6. RNase E, an RNA processing enzyme from Escherichia coli.
    J Biol Chem. 1979 Nov 10;254(21):11154-9 PMID: 387765
  7. Escherichia coli lac operator mRNA affects translation initiation of beta-galactosidase mRNA.
    Nature. 1979 Feb 1;277(5695):407-9 PMID: 121761
  8. Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331-5 PMID: 6158044
  9. Cloned structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12: localization on hybrid plasmid pTU100 and expression of a fragment of the gene.
    Mol Gen Genet. 1980;179(1):13-20 PMID: 6256604
  10. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
    Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
  11. Posttranscriptional control of bacteriophage lambda gene expression from a site distal to the gene.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):238-42 PMID: 6281759
  12. Purification and properties of ribonuclease E, an RNA-processing enzyme from Escherichia coli.
    Biochim Biophys Acta. 1983 Sep 28;747(3):200-8 PMID: 6351925
  13. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  14. mRNA processing in Escherichia coli: an activity encoded by the host processes bacteriophage f1 mRNAs.
    Nucleic Acids Res. 1984 Feb 24;12(4):1847-61 PMID: 6322124
  15. Growth-rate dependent regulation of mRNA stability in Escherichia coli.
    Nature. 1984 Nov 1-7;312(5989):75-7 PMID: 6387508
  16. Differential expression of photosynthesis genes in R. capsulata results from segmental differences in stability within the polycistronic rxcA transcript.
    Cell. 1985 Jan;40(1):171-81 PMID: 2981627
  17. Evidence that the 5' end of lac mRNA starts to decay as soon as it is synthesized.
    J Bacteriol. 1985 Feb;161(2):820-2 PMID: 3918023
  18. A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors.
    Gene. 1984 Nov;31(1-3):165-71 PMID: 6098521
  19. Maturation of Escherichia coli tryptophan operon mRNA: evidence for 3' exonucleolytic processing after rho-dependent termination.
    EMBO J. 1985 Jul;4(7):1887-91 PMID: 2992951
  20. Cloned DNA sequences that determine mRNA stability of bacteriophage phi X174 in vivo are functional.
    Nucleic Acids Res. 1985 Aug 26;13(16):5937-48 PMID: 2994019
  21. The stability of bacteriophage T4 gene 32 mRNA: a 5' leader sequence that can stabilize mRNA transcripts.
    Cell. 1985 Dec;43(2 Pt 1):461-9 PMID: 3000608
  22. Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1986 Jan;83(1):120-4 PMID: 2417233
  23. Identification of a positive retroregulator that stabilizes mRNAs in bacteria.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3233-7 PMID: 3085085
  24. The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments.
    Cell. 1986 Jul 18;46(2):245-51 PMID: 3521892
  25. Posttranscriptional control of Klebsiella pneumoniae nif mRNA stability by the nifL product.
    J Bacteriol. 1986 Oct;168(1):173-8 PMID: 2428807
  26. Induced mRNA stability in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1987 Jan;84(2):498-502 PMID: 3099297
  27. Stabilization of translationally active mRNA by prokaryotic REP sequences.
    Cell. 1987 Jan 30;48(2):297-310 PMID: 2433046
  28. Posttranscriptional regulation of ribosomal protein S20 and stability of the S20 mRNA species.
    J Bacteriol. 1987 Jun;169(6):2697-701 PMID: 2438268
  29. The first step in the functional inactivation of the Escherichia coli polynucleotide phosphorylase messenger is a ribonuclease III processing at the 5' end.
    EMBO J. 1987 Jul;6(7):2165-70 PMID: 3308454
  30. RNA processing by RNase III is involved in the synthesis of Escherichia coli polynucleotide phosphorylase.
    Mol Gen Genet. 1987 Aug;209(1):28-32 PMID: 2823071
  31. Differential mRNA stability controls relative gene expression within a polycistronic operon.
    Cell. 1987 Dec 24;51(6):1131-43 PMID: 2446776
  32. Processed mRNA with differential stability in the regulation of E. coli pilin gene expression.
    Cell. 1988 Jan 29;52(2):197-206 PMID: 2449283
  33. Site-specific endonucleolytic cleavages and the regulation of stability of E. coli ompA mRNA.
    Cell. 1988 Mar 25;52(6):893-901 PMID: 3280138
  34. In vivo and in vitro identity of site specific cleavages in the 5' non-coding region of ompA and bla mRNA in Escherichia coli.
    EMBO J. 1988 Jul;7(7):2269-75 PMID: 3046939
  35. Stabilization of discrete mRNA breakdown products in ams pnp rnb multiple mutants of Escherichia coli K-12.
    J Bacteriol. 1988 Oct;170(10):4625-33 PMID: 2459106
  36. A nuclease that cuts specifically in the ribosome binding site of some T4 mRNAs.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8895-9 PMID: 3057495
  37. Q-mediated late gene transcription of bacteriophage lambda: RNA start point and RNase III processing sites in vivo.
    Virology. 1988 Dec;167(2):568-77 PMID: 2849240
  38. Processing of unstable bacteriophage T4 gene 32 mRNAs into a stable species requires Escherichia coli ribonuclease E.
    EMBO J. 1988 Nov;7(11):3601-7 PMID: 3061803
  39. Erythromycin-induced stabilization of ermA messenger RNA in Staphylococcus aureus and Bacillus subtilis.
    J Mol Biol. 1988 Oct 20;203(4):905-15 PMID: 2463370
  40. Sequence changes preceding a Shine-Dalgarno region influence trpE mRNA translation and decay.
    J Mol Biol. 1988 Nov 5;204(1):51-60 PMID: 2464067
  41. Retroregulation of the bacteriophage lambda int gene: limited secondary degradation of the RNase III-processed transcript.
    J Bacteriol. 1989 Jan;171(1):588-92 PMID: 2521618
  42. A stable core region of the tra operon mRNA of plasmid R1-19.
    Nucleic Acids Res. 1989 Feb 25;17(4):1283-98 PMID: 2564189
  43. mRNA decay: finding the right targets.
    Cell. 1989 Apr 7;57(1):9-10 PMID: 2467747
  44. Transcriptional and posttranscriptional control of the Bacillus subtilis succinate dehydrogenase operon.
    J Bacteriol. 1989 Apr;171(4):2110-5 PMID: 2495271
  45. Mechanisms of mRNA decay in bacteria: a perspective.
    Gene. 1988 Dec 10;72(1-2):15-23 PMID: 3072246
  46. Purification and characterization of ribonuclease M and mRNA degradation in Escherichia coli.
    Eur J Biochem. 1989 May 1;181(2):363-70 PMID: 2653829
  47. Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12.
    J Bacteriol. 1989 Oct;171(10):5479-86 PMID: 2477358
  48. Autoregulation of RNase III operon by mRNA processing.
    EMBO J. 1989 Nov;8(11):3401-7 PMID: 2583104
  49. Transcription and messenger RNA processing upstream of bacteriophage T4 gene 32.
    Mol Gen Genet. 1989 Oct;219(1-2):39-48 PMID: 2615764
  50. Escherichia coli glyA mRNA decay: the role of 3' secondary structure and the effects of the pnp and rnb mutations.
    Mol Gen Genet. 1990 Jan;220(2):301-6 PMID: 1691434
  51. Degradation of tryptophan messenger. On the degradation of messenger RNA for the tryptophan operon in Escherichia coli.
    Nature. 1969 Jul 5;223(5201):37-40 PMID: 4893260
  52. Direction of in vivo degradation of tryptophan messenger RNA--a correction.
    Nature. 1969 Jul 5;223(5201):40-3 PMID: 4893261
  53. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-09-00
Pages
2731-41
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551980
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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